Life Technologies Corporation

Breg, Inc.

Paul Nelson

Shimon Spector

Syrus Jaffe

James M Fout

Jeffrey Regan

Philip Martin

David Bourdon

Tiffany A Bell

Lid of an electrophoresis tank

Electrophoresis comb with wedge-shaped teeth

A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. A lysis solution is passed through the filter in a second flow direction opposite the first direction, thereby dislodging the plurality of cells captured on the filter, and resuspending the plurality of cells in the lysis solution on the first side of the filter. The lysis solution lyses the plurality of cells to produce a cellular lysate. The cellular lysate is passed through the filter in the first flow direction.

Filter based system for capture of lysis of suspended cells

Electrophoresis cassette base

Electrophoresis tank with a base and cassette inserted in

Electrophoresis tank

An orthopedic walker boot for wearing on a lower limb of a wearer has a rigid external shell, a pliant internal boot positioned in the external shell, and a heel plate selectively detachable or attachable to the external shell. Selective transition of the heel plate between the detached condition and attached condition selectively provides the orthopedic walker boot with an open-heel configuration or a closed-heel configuration. The external shell includes an outer sole having a walking surface which is configured with two curved axes to facilitate walking while wearing the orthopedic walker boot.

Orthopedic walker boot having a removable heel plate

Growing community of inventors

Encinitas, CA, United States of America

Paul Nelson