Abbott Laboratories Corporation

Ensign-bickford Aerospace & Defense Company

Paul N. Marshall

Paul N Marshall

Craig Boucher

David B Novotney

Luc Van Hove

John G Glazier

Anne M Embleton

Robert M Studholme

Whole blood is mixed with a reticulocyte reagent system that has a reticulocyte staining reagent and a diluent reagent, used in combination. This mixture is incubated at room temperature for between about 15 minutes to about 4 hours. The incubated mixture is then analyzed and the light scattering properties of the cells are detected, collected, differentiated and quantitized. Data gathering includes, at least, 10 and 90 degree light scatter detection.

Reagent system and method for the differentiation and identification of

A lytic agent is described which when mixed with whole blood, lyses the red cells instantly, but does not affect the optical, light scattering properties of the white blood cells for at least 30 seconds. This lytic agent enables the performance of a five part leukocyte differential by flow cytometry and light scattering. In its preferred embodiment, the lytic agent incorporates 2-phenoxyethanol which combines the functions of leukoprotective and antimicrobial, TRITON X-100 (octylphenoxypolyethoxyethanol) a lytic and wetting agent and TRIS/HCl to provide pH buffering and to increase the solution's conductivity.

Flow cytometry lytic agent and method enabling 5-part leukocyte

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Santa Clara, CA, United States of America

Paul N Marshall