Kapa Biosystems, Inc.

Paul McEwan

William Bourn

John Foskett

Eric Van Der Walt

Gavin Rush

Martin Ranik

Maryke Appel

Bjarne Faurholm

Wolfgang Schafer

Jennifer Hsieh

Ross Wadsworth

Marliz Strydom

Gerrida Uys

Abre De Beer

Disclosed is a method of fragmenting DNA comprising contacting a sample of target DNA with (a) a composition comprising an active transpososome, and (b) a composition comprising an inactive transpososome, under conditions suitable for transpososome activity, wherein a ratio of an amount of the inactive transpososome in the composition of (b) to an amount of the active transpososome in the composition of (a) determines the mean fragment size and a level of insertion bias.

Transposase competitor control system

The present invention provides improved DNA polymerases, in particular, type A DNA polymerases, that may be better suited for applications in recombinant DNA technologies. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.

Modified type A DNA polymerases

The disclosure provides a composition comprising a double-stranded deoxyribonucleic acid (dsDNA) sequence comprising from 5' to 3&#x2032;, a sequence comprising a first adaptor sequence, a template sequence, and a second adaptor sequence, wherein the second adaptor sequence comprises a hybridization site for a template switching oligonucleotide (TSO). The disclosure provides methods for making the compositions of the disclosure using a template switching mechanism to add non-templated basepairs to the ends of a DNA molecule, hybridize a TSO to the non-templated basepairs, and then extend the sequence complementary to the TSO to add an adaptor.

System and method for nucleic acid library preparation via template switching mechanism

The present invention provides synthetic nucleic acid molecule tags that can be added into samples for identification and tracking. Among other things, the present invention provides synthetic, high-molecular weight concatemers, which can be combined with samples to yield hundreds of millions of unique identifiers. Examples applications for which the synthetic nucleic acid molecule tags can be used, include industrial, research and clinical applications.

High-molecular weight DNA sample tracking tags for next generation sequencing

The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.

Modified DNA polymerases for improved amplification

The present invention provides, among other things, chimeric DNA polymerases containing heterologous domains having sequences derived from at least two DNA polymerases that have at least one distinct functional characteristics (e.g., elongation rate, processivity, error rate or fidelity, salt tolerance or resistance) and methods of making and using the same. In some embodiments, the present invention can combine desired functional characteristics (e.g., high processivity; high elongation rate; thermostability; resistance to salt, PCR additives (e.g., PCR enhancers) and other impurities; and high fidelity) of different DNA polymerases in a chimeric polymerase.

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Western Cape, South Africa

Paul McEwan