Children's Medical Center Corporation

Syntex (u.s.a.) Inc.

Children's Hospital Medical Center

University of Pittsburgh

Paul M Gallop

Donald R Korb

Mercedes A Paz

Paul A Rosenberg

Elias Aizenman

Kenneth H Gabbay

Robert J Levy

Rudolf Fluckiger

Edward Henson

Peter V Hauschka

Helina Borel

B David Stollar

Yves Borel

Jane B Lian

PQQ and PQQ-generating compounds inhibit osteoclast cell formation and bone resorption, and those compounds can be used to treat medical conditions characterized by undesired bone resorption. PQQ-phosphonates that target osteoclasts at the bone-mineral interface are particularly useful in this regard.

Controlling bone resorption with pyrroloquinoline quinone (PQQ) and

Methods are disclosed for treating PQQ-responsive heavy metal toxicity which include the administration of PQQ to a patient in need thereof. The toxicity may be associated with such heavy metals as lead, indium and vanadium.

Method for treating PQQ-responsive heavy metal toxicity

A method of resisting neurological damage caused by overstimulation of the NMDA receptor of nerve cells by glutamate includes exposing the NMDA receptors to an oxidizing agent to thereby diminish overall activity of the receptors following activation by glutamate. The oxidizing agent preferably is a material selected from the group consisting of pyrroloquinoline quinone and topa hydantoin.

Method of resisting neurodegenerative disorders

A method for resisting neurological damage caused by overstimulation of the MDA receptor of nerve cells by glutamate includes exposing the NMDA receptors to an oxidizing agent to thereby diminish overall activity of the receptors following activation by glutamate. The oxidizing agent preferably is a material selected from the group consisting of pyrroloquinoline quinone and topa hydantoin.

A method of forming analyzable adducts in a mixture of compounds by contacting the mixture with a boron reagent having the formula of either ##STR1## where each X and Y is, independently, an alkyl group of 12 or fewer carbons or an aryl group of 6-20 carbons; or BZ.sub.3, where each Z is, independently, an alkyl group of 12 or fewer carbons, or an aryl group of 6-20 carbons.

Formation of analyzable boron containing adducts

Compounds and methods featuring, in one aspect, compositions containing an organic boronic acid and one or more reporter groups.

Reporter compounds containing boron

Conjugate composed of an oligonucleotide covalently bonded to a carrier.

Oligonucleotide conjugates

Copolymers having improved machining and water resistant properties are produced using a monomer containing an adduct protecting group, particularly an alkyl boronic acid adduct, of glyceryl acrylate or glyceryl methacrylate, in combination with an alkyl acrylate or methacrylate, and optionally a glycidyl acrylate or methacrylate. After machining and shaping, the adduct is removed to provide a hydrogel useful as a contact lens material.

Copolymers and hydrogels: process and articles made thereof

The invention provides new hydrogel forming materials comprising synthetic, hydrophilic, aqueous-insoluble polymer networks formed by copolymerization of a hydrophilic dihydroxyalkyl acrylate or methacrylate, a substantially water insoluble alkyl acrylate or methacrylate, one or more additional hydrophilic comonomers selected from the group of vinylic monomers, acrylates, and methacrylates, and a cross-linking agent. The hydrogels are preferably used for the formation of contact lenses, but may also be used for drug and pesticides delivery devices; dialysis, ultrafiltration and reverse osmosis membranes; implants in surgery and dentistry; and the like.

Polymeric compositions and hydrogels formed therefrom

Reporter compounds

A method of measuring glycosylated hemoglobin featuring, in one aspect, providing a sample containing hemoglobin, including an unknown amount of glycosylated hemoglobin, contacting the sample with an oxidizing agent to generate formaldehyde, and measuring the formaldehyde as a measure of glycosylated hemoglobin, the measuring being carried out by reacting the formaldehyde with a water-soluble amine or an ammonium salt and a .beta.-diketone to generate a fluorescent compound whose fluorescence is measured as a measure of glycosylated hemoglobin.

Glycohemoglobin determination

Compositions and processes featuring, in one aspect, a process of purifying a protein diagnostic for atherosclerosis comprising separating out of primate and human atherosclerotic tissue a Gla-containing protein of molecular weight of about 80,000.

Protein diagnostic for atherosclerosis

The invention provides a method of treating hydrogels formed from the polymerization of monomers having reactive olefinic double bonds by contacting them with reactive thiol compounds. In a preferred embodiment where the hydrogel structure includes a cis-glycol containing unit, a suitable thiol is used in conjunction with a borate buffer. The present invention also provides materials that are treated with the above thiol compounds.

Growing community of inventors

Chestnut Hill, MA, United States of America

Paul M Gallop