The J. David Gladstone Institutes

Other

Paul Klenerman

Douglas F Nixon

Martin Bigos

Megan Sheehy

Adrian B McDermott

Scott Furlan

Patrice Andre

Vincent Lotteau

Rolf Zinkernagel

Marcus Groettrup

The present invention provides methods of detecting specific lysis of a cell by a lytic agent. The methods generally involve contacting a labeled target cell with a lytic agent; and detecting fluorescence in the target cell. The target cells are labeled with two fluorescent labels: a first fluorescent label that labels the plasma membrane; and a second fluorescent label that labels the cytosol. Release of the cytosolic label from the target cell indicates that the target cell has been lysed. The invention further provides methods of detecting the presence in a sample of a cell that specifically lyses a target cell. The invention further provides methods of detecting the presence in a sample of an antibody that specifically lyses a target cell. The methods are useful in a variety of applications.

Methods of detecting specific cell lysis

The invention relates to the use of at least one compound that inhibits HIV (human immunodeficiency virus) protease, selected from ritonavir, saquinavir or one of the pharmaceutically acceptable salts thereof, in association with a pharmaceutically acceptable vehicle for the production of a medicament to modulate proteasome.

Use of HIV protease inhibiting compounds

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Oxford, United Kingdom

Paul Klenerman