Dna Plant Technology Corporation

Advanced Genetic Sciences, Inc.

Paul K Wolber

Robert L Green

Neal I Gutterson

William T Tucker

Gareth J Warren

Viable bacteria may be detected in biological samples by exposing bacterial cultures obtained from the samples to transducing particles having a known host range. Such transducing particles carry a heterologous gene capable of altering the phenotype of the bacteria in a readily detectable manner. For example, the transducing particles may carry an ice nucleation gene and the alteration of phenotype may be detected using an ice nucleation assay. By employing a panel of phage, unknown bacteria may be typed based on the pattern of reactivity observed. The method is particularly useful for detecting viable bacteria which may have been debilitated by exposure to sterilizing conditions, such as in food processing. The method is also useful for tracking a target bacteria in the ambient environment.

Bacterial detection by phage transduction of ice nucleation and other

Viable bacteria may be detected in biological samples by exposing bacterial cultures obtained from the samples to transducing particles having a known host range. Such transducing particles carry a heterologous gene capable of altering the phenotype of the bacteria in a readily detectable manner. For example, the transducing particles may carry an ice nucleation gene and the alteration of phenotype may be detected using an ice nucleation assay. By employing a panel of phage, unknown bacteria nmay be typed based on the pattern of reactivity observed. The transducing particles may be prepared by introducing a synthetic transposable element carrying the heterologous gene to a host carrying a prophage having the desired host range. After transposition, the host may be induced to a lytic cycle to release the transducing particles carrying the heterologous gene.

Transducing particles and methods for their production

A novel method for carrying out immunoassays is described. The method involves use of a biological ice nucleating agent as a label. In particular, the method involves use of ice nucleating bacteria, or ice nucleating components derived therefrom, as a label.

Growing community of inventors

Hayward, CA, United States of America

Paul K Wolber