Pierce Biotechnology, Inc.

Paul Jeffrey Haney

Christopher Laurence Etienne

Ramesh Ganapathy

Erum Raja

Sijian Hou

Michael Robert Johnston

Jeffery James Frank Gordon

William Klungle

Brendan George Metz

Navid R Haghdoost

Mike Robert Johnston

Peptide and/or protein quantitation methods, kits, and compositions, particularly useful for mass spectrometry, are provided herein based on a bathocuproine-based composition complex such as bathocuproinedisulfonic acid disodium salt hydrate complex. The methods are one-step rapid absorbance methods using small sample volumes. They produce a robust signal with high signal to background ratio and accurately quantitate even complex peptide mixtures with low variability and high sensitivity.

Quantitative peptide or protein assay

A dialysis device, and a method of dialyzing a sample, are provided. The dialysis device includes a body having a first and a second major side, a first and a second retaining ring disposed on the first and second major sides, and a first and second dialysis membrane disposed, and held between the first and second major sides and the first and second retaining rings. The dialysis device is configured to receive a sample to enable dialysis of the sample across the membranes.

Dialysis device

A device for dialysis of a sample includes a sample chamber formed by a gasket and dialysis membranes affixed to each side of the gasket in facing relationship. A housing supports the dialysis membranes and gasket. An air chamber causes the device to float in a generally upright position when the device is immersed in a dialysate. An access port is provided through the gasket and between the dialysis membranes. The access port is accessible from outside the housing and in communication with the sample chamber such that a dispensing end of a dispensing device can be inserted through the access port to dispense sample into the sample chamber. In one embodiment, the gasket is impermeable or essentially impermeable to the sample being dialyzed.

Dialysis device with access port

A device for the dialysis of a sample includes a hermetically sealed sample chamber formed by a gasket with dialysis membranes affixed to each side in facing relationship. The gasket is impermeable to the sample being dialyzed, but is penetrable and reusable such that a sample introduction mechanism can be inserted through the gasket into the chamber, and then withdrawn without sample being permitted to leak. The device is fitted into a rigid housing containing windows and at least one port parallel to the dialysis membranes for directing the sample introduction mechanism into the gasket. The housing includes at least one pressure ridge for assisting in hermetically sealing the sample chamber and air chamber at one end for causing the device to float in an upright position when immersed in a dialysate.

Dialysis device with air chamber

Growing community of inventors

Beloit, WI, United States of America

Paul Jeffrey Haney