University of Pittsburgh

The Charles Stark Draper Laboratory, Inc.

Cellomics, Inc.

Other

Tha Charles Stark Draper Laboratory, Inc.

Paul J Sammak

Rami S Mangoubi

Mukund Desai

Gustavo Rosania

Nathan Lowry

Richard A Rubin

Teresa M Erb

Kim Ippolito

Ravi Kapur

Christopher Jeffreys

Lawrence J Zana

Jason Bush

Alex Friedman

Sarah Burroughs Tencza

Oleg Lapets

Randall Shopoff

Michel Nederlof

Murugan Kannan

Thomas D Duensing

Sara Mucko

Methods for inducing differentiation of stem cells into trophectoderm, neurectoderm, or progeny cells thereof are described, and mathematical and statistical image analysis methods and systems, for example, to identify differentiated cells and screen for agents which modulate differentiation are provided for separate or combined use.

Methods of generating trophectoderm and neurectoderm from human embryonic stem cells

Mathematical and statistical image analysis methods and systems are applied to enhance and refine the process of reprogramming cells, for example, to modify cells from patients into custom-matched stem cells.

Mathematical image analysis based cell reprogramming with applications for epigenetic and non-epigenetic base induced pluripotent stem cell derivation

The invention provides methods for determining the differentiation state of cells. The methods include non-invasive, non-perturbing, automatable, and quantitative methods of analysis of cell colonies, individual cells, and/or cellular structures.

Image-based methods for measuring global nuclear patterns as epigenetic markers of cell differentiation

The present invention provides automated methods and associated software for determining the organization of a component of interest in individual cells by determining the amount or distribution of the cellular component of interest as a function of position relative to a reference component in the individual cells.

Methods for determining the organization of a cellular component of interest

The invention generally provides methods and systems for determining characteristics of cellular structures. The methods include non-invasive, non-perturbing, automatable, and quantitative methods and may be applied to the examination of cells such as stem cells, embryos, and egg cells.

Methods and systems for imaging cells

The present invention provides a test plate and methods for adjusting fluorescence imaging systems involving using a plate with fluorescent microbeads bound to a surface.

Microbead-based test methods for fluorescence imaging systems

The present invention provides methods and software for tracking individual cells during a kinetic cell screening assay.

Automated assay for identification of individual cells during kinetic assays

Microbead-based test plates and test methods for fluorescence imaging systems

The present invention provides automated systems, methods, screens, and software for the analysis of cell spreading. The invention involves providing cells containing fluorescent reporter molecules in an array of locations, contacting the cells with a test stimulus, acquiring images from the cells, and automatically calculating one or more morphological features that provide a measure of cell spreading.

System for cell-based screening

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Pittsburgh, PA, United States of America

Paul J Sammak