Schering Corporation

Schering-plough Corporation

Paul J Leibowitz

Yair Alroy

Marvin J Weinstein

Michael J Ryan

Satwant K Narula

Richard L Reim

Extraction of granulocyte macrophage colony stimulating factor from

Expression systems which utilize bacteriophage T7 RNA mutant polymerases and promoter regions are provided by this invention. These systems are utilized to produce large quantities of useful polypeptides and proteins.

Expression systems utilizing bacteriophage T7 promoters, gene sequences,

A method is provided for extracting human interleukin-4 (IL-4) from IL-4 expressing bacterial cells. The method comprises treating the bacterial cells with a deactivating agent, disrupting the cell membrane and recovering the IL-4.

Extraction of human interleukin-4- from bacteria

A method of extracting granulocyte macrophage colony stimulating factor (GM-CSF) from GM-CSF-expressing bacterial cells comprising treating a suspension of GM-CSF-containing bacterial cells with an acid and an enhancing agent, removing substantially all of the suspension liquid from the cells, preparing a second suspension of the acidified cells, neutralizing said second suspension, and separating the GM-CSF containing liquid from the suspended cells.

The invention features a novel hybrid interferon species that comprises a chain of 161 and/or 162 amino acids. The hybrid is novel not only for its new structure, but also because the hybrid comprises a shortened or truncated segment of alpha interferon, and hence, an entirely new interferon species which is not occurring in nature.

Novel hybrid interferon species

There are disclosed compositions and methods for treating tumors and viruses in humans by administering a combination of gamma interferon and delta-4 alpha-2 (Bgl II-1)/(Bgl II) alpha-1 hybrid interferon, preferably by injection.

Interferon combinations

A method of extracting leucocyte interferon from interferon-expressing bacterial cells comprising acidifying a suspension of interferon-containing bacterial cells, removing substantially all of the suspension liquid from the cells, preparing a second suspension of the acidified cells, neutralizing said second suspension, separating the interferon containing liquid from the suspended cells, and extracting the interferon from said liquid.

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Hackensack, NJ, United States of America

Paul J Leibowitz