Emd Millipore Corporation

University of California

Emd Millipore Corporaticn

Paul J Hung

Philip Janmin Lee

Luke P Lee

Phillip J Lee

Microfluidic devices, systems, and methods providing for an invasion assay using microfluidic culture systems.

Cell culture and invasion assay method and system

Methods and systems are described for improved handling and/or culturing and/or assaying of cells, chemically active beads, or similar materials in microfluidic systems and microfluidic culture arrays.

Methods and apparatus for cell culture array

A number of novel improved microfluidic configurations and systems and methods of manufacture and operation. In one embodiment, three wells are used for independent cell culture systems in a cell culture array. In a second aspect, artificial sinusoids with artificial epithelial barriers are provided with just one (optionally shared or multiplexed) fluidic inlet and one (optionally shared or multiplexed) fluidic output, where the medium output also functions as a cellular input. A pneumatic cell loader combined with other components provides a fully automated cell culture system. Magnetic alignment of plate molds provides advantages and ease of molded manufacture.

Cell culture array system for automated assays and methods of operation and manufacture

In one embodiment, a microfluidic structure comprises a culture chamber having an object flow inlet disposed between a pair of object flow outlets. A flow-around channel provides fluidic mass transport to the culture chamber through a perfusion barrier disposed opposite from the object flow inlet and object flow outlets. The perfusion barrier surrounds the culture chamber, defines an opposite wall of the culture chamber, and prevents cell passage into the flow around-channel. The perfusion barrier creates a low fluidic resistance path within the culture chamber, such that a flow of cells entering the culture chamber from the object flow inlet encounters a flow of media passing through the perfusion barrier. This causes the cells to take an approximately 180 degree turn and exit the culture chamber via the object flow outlets. The low fluidic resistance path allows the cells to settle onto the chamber floor without needing any physical barrier.

Microfluidic cell culture systems

Cell culture array system for automated assays and methods of operation and manufacture thereof

Method and systems that provide improved handling and/or culturing and/or assaying of cells, chemically active beads, or similar materials in microfluidic systems and microfluidic culture arrays.

Growing community of inventors

Berkeley, CA, United States of America

Paul J Hung