University of Washington

Phaserx, Inc.

Genevant Sciences Gmbh

Marina Biotech, Inc.

Nastech Pharmaceutical Company, Inc.

Paul Hickok Johnson

Allan Sachs Hoffman

Patrick S Stayton

Anthony Convertine

Robert W Overell

Anna S Gall

Craig L Duvall

Charbel Diab

Priyadarsi De

Mary Gallagher Prieve

Danielle Benoit

Amber E E Paschal

Steven C Quay

Michael E Houston, Jr

Richard E Herman

Sean D Monahan

Michael S DeClue

Douglas L Badders

Scott M Henry

Amber E Paschal

Amber Ee Paschal

Provided herein are micellic assemblies comprising a plurality of copolymers. In certain instauces, micellic assemblies provided herein are pH sensitive particles.

Micellic assemblies

Polymeric compounds having spatially controlled bioconjugation sites are described. Functionalization is achieved by selective &#x3c9;-terminal chain extension of polymer chains by radical polymerization, such as reversible addition-fragmentation chain transfer (RAFT) polymerization.

&#x3c9;-functionalized polymers, junction-functionalized block copolymers, polymer bioconjugates, and radical chain extension polymerization

Described herein are copolymers, and methods of making and utilizing such copolymers. Such copolymers have at least two blocks: a first block that has at least one unit that is hydrophilic at physiologic pH, and a second block that has hydrophobic groups. This second block further has at least one unit with a group that is anionic at about physiologic pH. The described copolymers are disruptive of a cellular membrane, including an extracellular membrane, an intracellular membrane, a vesicle, an organelle, an endosome, a liposome, or a red blood cell. Preferably, in certain instances, the copolymer disrupts the membrane and enters the intracellular environment. In specific examples, the copolymer is endosomolytic.

Diblock copolymers and polynucleotide complexes thereof for delivery into cells

Provided herein are micellic assemblies comprising a plurality of copolymers. In certain instances, micellic assemblies provided herein are pH sensitive particles.

Omega-functionalized polymers, junction-functionalized block copolymers, polymer bioconjugates, and radical chain extension polymerization

Provided herein are multiblock copolymers, as well as micelles and therapeutic compositions thereof.

Multiblock copolymers

Provided herein are monomers incorporating folate or other targeting agent, polymers prepared therefrom, polymers prepared therefrom having a therapeutic agent covalently coupled thereto, as well as micelles and therapeutic compositions thereof.

Targeting monomers and polymers having targeting blocks

A composition for delivering an agent to a cell, comprising a bispecific affinity reagent and a pH-responsive, membrane destabilizing polymer. The bispecific affinity reagent may include a first affinity reagent covalently linked to a second affinity reagent, wherein the first affinity reagent binds to a molecule on the surface of a cell, and the second affinity reagent binds to an intracellular target.

Bispecific intracellular delivery vehicles

Compositions comprising a heterogeneous polymeric micelle and an agent (e.g., a polynucleotide) associated with the micelle are disclosed, together with methods for intracellular delivery of such agent.

Heterogeneous polymeric micelles for intracellular delivery

Provided herein are polymeric carriers suitable for the delivery of polynucleotides (e.g. oligonucleotides) and/or other therapeutic agents into a living cell.

Polymeric carrier

Trp cage binding domains polypeptides are disclosed. The Trp cage binding domains have the generic formulae of SEQ ID NO: 2, 7, 10 or 11. They can be efficiently produced and screened using phage display technology.

Growing community of inventors

Snohomish, WA, United States of America

Paul Hickok Johnson