Washington University

University of Pittsburgh

The University of Chicago

The United States Government As Represented by the Department of Veterans Affairs

Paul H Schlesinger

Samuel A Wickline

Gregory M Lanza

Neelesh R Soman

Stanley J Korsmeyer

George W Gokel

Hua Pan

Harry Colbert Blair

Deborah J Nelson

Quitterie Larrouture

Irina L Tourkova

Neelesh Soman

Provided herein are methods of producing trabecular bone material, such as trabecular bone or trabecular bone matrix and cells useful in preparing the trabecular bone material.

Method of making trabecular bone

The present invention provides a substantially non-lytic, non-cytotoxic anchor peptide that is capable of stably inserting into lipid membranes. In particular, the invention provides nanoparticles comprising stably inserted anchor peptides, which may be conjugated to a variety of different cargo complexes.

Universal anchor peptide for nanoparticles

Compositions which comprise emulsions of nanoparticles for delivery of membrane-integrating peptides are described. The nanoparticles comprise a liquid hydrophobic core coated with a lipid/surfactant layer which contains the membrane-integrating peptides. Methods to use such compositions are also described.

Nanoparticle delivery systems for membrane-integrating peptides

Nanoparticle delivery systems comprising a hydrophobic core and a lipid/surfactant layer comprising a membrane-lytic peptide

The present invention relates to amphiphiles for the formation of a synthetic ion channel in a phospholipid bilayer or biomembrane. The synthetic channel selectively transports chloride ions across the phospholipid bilayer or the membrane. Said amphiphiles impart ion permeability to the phospholipid bilayers or biomembrane and also modulate cellular volume in mammalian cells in vitro.

Synthetic ion channels

Methods for identifying apoptosis-modulating compounds using lipid bilayers are provided. One method involves contacting a compound of interest with a lipid bilayer which contains an ion-channel formed by an anti-apoptotic or pro-apoptotic polypeptide of the BCL-2 family and assaying for changes in the ion conductance properties of the channel, including ion selectivity, single channel conductance and rectification. A second method identifies compounds which can form ion channels in planar lipid bilayers and determines the ion selectivity and pH dependence of such channel formation, where apoptosis modulating activity is predicted based on comparing these channel forming characteristics with those of Bcl-2 family members.

Method for identifying apoptosis modulating compounds

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St. Louis, MO, United States of America

Paul H Schlesinger