Biometric Imaging, Inc.

Paul G Hayter

Bala S Manian

Robert S Dubrow

Thomas Michael Baer

Robert Justice Shartle

Vartan E Ghazarossian

Louis John Dietz

Michael Hodges

Analytes can be detected from among closely related substances by reacting the analyte in a test sample with a labeled binding agent which specifically binds to the analyte to form a complex. The labeled binding agent is supplied in excess, and the complex is identified through a time window relative to the detection of the excess unbound agent. The complex and labeled binding agent are isolated on a separation media and identified by the differential rate of migration.

Differential separation assay methods and test kits

The apparatus and method of the present invention disclose a scanning imaging cytometer wherein an unprocessed biological fluid sample is reacted with a fluorescently-labeled binding agent. The reacted sample undergoes minimal processing before it is placed into a capillary tube. The sample is optically scanned and fluorescence excitation is recorded from a plurality of columnar regions of the capillary tube, each columnar region generally defined by the spot size of the excitation beam and the depth dimension of the capillary tube. A spatial filter of a sufficient pinhole diameter is selected to allow simultaneous volumetric detection of all fluorescent targets in each columnar region.

Apparatus and method for volumetric capillary cytometry

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Los Altos, CA, United States of America

Paul G Hayter