Wisconsin Alumni Research Foundation

Hyradix Incorporated

Paul G Blommel

Kurt M Vanden Bussche

Suheil F Abdo

Bradley P Russell

Brian Fox

Robert J Sanger

Warren Lyman

John R Harness

Daniel R Sioui

Randy D Cortright

Li Yuan

The invention discloses a method of determining protease activity, in real time, using fluorescence polarization technology. In particular, the invention provides vectors and a method for their use, which expresses uncharacterized proteins conjugated to a fluorescence tag, which binds specifically to a fluorescent ligand. Cleavage of the recombinant protein results in a fragment of the expressed peptide and results in a change in fluorescence polarization of the fluorophore. The rate of change in fluorescence polarization can be measured in real time and is equivalent to the rate of protease cleavage.

Fluorescence polarization assay to detect protease cleavage

Hydrogen generators and integrated hydrogen generator/fuel cells systems are operated by determining the condition of the hydrogen generator and the condition of the fuel to the hydrogen generator for selection of predetermined flow rates for each of the externally-provided raw materials. The processes of the invention can provide rapid transitions between hydrogen production rates while enabling enhanced efficiency and stability during transient operations.

Feedforward control processes for variable output hydrogen generators

Growing community of inventors

Blue Mounds, WI, United States of America

Paul G Blommel