Miles Inc.

Bayer Corporation

Miles Laboratories, Inc.

Other

Bayer Healthcare LLC

Siemens Healthcare Diagnostics Gmbh

Corey, Paul F.

Paul F Corey

Michael J Pugia

Gary E Rehm

Matthew K Musho

Steven W Felman

Ronald G Sommer

Kin F Yip

Meitak Teresa Yip

Frederick E Ward

M Teresa Yip

Angela A Michaels

Michael L Bloczynski

Harold C Kluender

Hans-Ulrich Siegmund

Michael Salvati

Yingping Deng

A Christopher Skjold

Alison J Murray

James H Pendergrass

Lonnie R Stover

Lois J Proud

Robert W Trimmer

Thomas Boecker

Kin Fai Yip

Disclosed is an assay for determining the presence and concentration of trypsin inhibitor in urine samples. The assay reagents, which may be used either in the liquid or dry states, include trypsin, a trypsin substrate and a polycarboxylic chelating agent. The inclusion of the chelating agent in the assay has been found to reduce variation in the assay results.

Urinary trypsin inhibitor assay containing a chelating agent

A compound of the formula (I)wherein Ris a protecting group for N&#x3b1;, Ris a protecting group for N, and Ris aryl, and wherein the compound of formula (I) is a trypsin substrate such that trypsin cleaves the O&#x2014;C single bond, which liberates R&#x2014;OH; a diagnostic device comprising same; a method for preparing the diagnostic device; and a method of using the diagnostic device to detect levels of urinary trypsin inhibitor in a biological sample; and a diagnostic kit for detecting levels of urinary trypsin inhibitor in a biological sample.

Trypsin substrate and diagnostic device, and method of using same

An improved test strip for determining the presence or concentration of unknown or a constituent in a liquid test sample is disclosed. The test strip comprises a support strip and a test pad, wherein the test pad includes a carrier matrix incorporating a reagent composition capable of interacting with the constituent of interest to produce a detectable or measurable response. The test strip further comprises an infrared dye, applied either to the support strip or incorporated into a test pad, which ensures proper alignment of the test strip in an apparatus having a detection system for the detectable or measurable response. The improved test strip reduces the number of erroneous assays for the constituent of interest.

Test strip for the assay of an analyte in a liquid sample

Disclosed is an improved electrode suitable for the electrochemical regeneration of the co-enzymes NADH and NADPH. The electrode has imparted on its surface a mediator function which is a 3-phenylimino-3H-phenothiazine or a 3-phenylimino-3H-phenoxazine. Also disclosed is a method of improving the performance of a biochemical fuel cell which operates with a dehydrogenase as a catalyst and a co-enzyme as the energy-transferring redox couple which involves using the improved electrode in the fuel cell.

Mediators suitable for the electrochemical regeneration of NADH, NADPH

Disclosed is an electrode suitable for the electrochemical regeneration of the co-enzymes NADH and NADPH. The electrode has imparted on its surface a mediator function which is a 3-methylene-3H-phenothiazine or a 3-methylene-3H-phenoxazine compound.

Electrode mediators for regeneration of NADH and NADPH

Disclosed is the use of 9H-acridin-2-one and 11H-dibenz-[b,f][1,4]oxazepin-8-one compounds as mediators suitable for the electrochemical regeneration of the co-enzymes dihydronicotinamide adenine dinucleotide (NADH), dihydronicotinamide adenine dinucleotide phosphate (NADPH) or analogs thereof.

The present test device provides a merocyanine protein error indicator compound. Merocyanine compounds are a new class of protein error indicators, providing an analytical tool useful in the detection of protein in a sample.

Merocyanine protein error indicators

Disclosed are arylazo chromoionophores characterized by the formula: ##STR1## wherein X is hydrogen or a monovalent cation, Y is H or methoxy and R is a ringed aromatic organic structure which affects the optical absorption properties of the compounds. Also disclosed is a method for the detection of calcium ion using these compounds.

Arylazo chromoionophores

The present invention provides an analytical test strip for the detection of protein in a biological sample including a novel combination of protein error indicators. The invention includes, in combination, a partially halogenated phenolsulfonephthalein protein error indicator having nitro or nitroso substituent groups in the B and/or C rings and a merocyanine protein error indicator.

Merocyanine and nitro or nitroso substituted polyhalogenated

The present invention provides a merocyanine protein error indicator compound. Merocyanine compounds are a new class of protein error indicators, providing an analytical tool useful in the detection of protein in a sample.

Disclosed are 8-Hydroxy-2H-dibenz[b,f]azepin-2-one dyes, chromogenic enzyme substrates made from them and methods for preparing the same.

8-hydroxy-2H-dibenz(b,f)azepin-2-one dyes

Chromogenic merocyanine enzyme substrate compounds of the general formula: ##STR1## where Y is an enzymatically-cleavable group such as a radical of a sugar, carboxylic acid, amino acid, peptide, phosphoric acid, or sulfuric acid; A and B represent residues that complete 5- or 6-membered ring systems; R.sup.1 is substituted or unsubstituted alkyl; R.sup.2 and R.sup.3, independently, are hydrogen or lower alkyl; m, n, and p, which can be different, are integers from 0 through 3 provided that m+n+p must be at least 2; and X is an appropriate counterion (anion).

Chromogenic merocyanine enzyme substrates

The present invention provides a novel group of protein error indicators including nitro or nitroso substituent groups in the B and C rings of a partially halogenated triphenylmethane compound.

Nitro or nitroso substituted polyhalogenated phenolsulfonephthaleins as

Chromogenic enzyme substrate compounds comprising a dibenz[b,e] [1,4]oxazepinone or dibenzo[b,e] [1,4]thiazepinone nucleus having an enzyme-cleavable group such as a radical of a sugar, carboxylic acid, amino acid, peptide, phosphoric acid, or sulfuric acid. The substrate compounds are, in general, highly soluble in aqueous media and only slightly colored, and produce, upon enzyme cleavage, a chromogen exhibiting a large change in absorbance and a pKa below 7. Such substrates find use as indicators for the determination of enzyme analytes and enzymes used as markers in a variety of assays, including immunoassays.

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Elkhart, IN, United States of America

Paul F Corey