Olin Corporation

Paul E Holmes

Katherine P Roberts

Christine M August

Jon A Kornacki

This invention is directed to a process for providing enhanced yield and simplified isolation of hydrophobic enzymes from a culture medium. The process comprises the steps of: (a) culturing a hydrophobic enzyme-secreting microorganism in an aqueous culture medium comprising a yield-enhancing effective amount of a nonionic surfactant having a cloud point of less than 40.degree. C. for a time sufficient to provide enhanced secretion of said enzyme from said microorganism, (b) removing said microorganism from said culture medium to provide a microorganism-free solution, (c) heating said microorganism-free solution to a temperature above said cloud point to cause phase separation of said culture medium into an aqueous phase, and a non-aqueous phase containing said nonionic surfactant and said yield-enhanced hydrophobic enzyme, and (d) isolating said non-aqueous phase containing said nonionic surfactant and said yield-enhanced hydrophobic enzyme.

Process for providing enhanced yield and simplified isolation of

A novel lipase from a newly-discovered strain of Pseudomonas alcaligenes microorganism having (i) an optimum pH for activity of about 10.+-.0.5; (ii) an optimum temperature for activity of about 45.degree. to 55.degree. C.; (iii) an optimum pH for stability of about 7.0.+-.0.5; (iv) a molecular weight as measured by SDS-PAGE of about 3.0.times.10.sup.4 ; and (v) chemical stability for at least a 60 day mean half-life in the presence of a 10 percent solution of polyoxyethylene (23) lauryl ether in 25 millimolar aqueous calcium chloride. Also claimed is a biologically pure culture of the microorganism, and a method for the production of the lipase. Also claimed is a lipase characterized by containing an N-terminal amino acid sequence which is Gly-Leu-Phe-Gly-Pro-Ser-Gly-Tyr-Thr-Lys-Thr-Lys-Tyr-Pro-Ile.

Identification, characterization and method of production of a novel

A novel lipase from a newly-discovered strain of Pseudomonas alcaligenes microorganism having (i) an optimum pH for activity of about 10.+-.0.5; (ii) an optimum temperature for activity of about 45.degree. to 55.degree. C.; (iii) an optimum pH for stability of about 7.0.+-.0.5; (iv) a molecular weight as measured by SDS-PAGE of about 3.0.times.10.sup.4 ; and (v) chemical stability for at least a 60 day mean half-life in the presence of a 10 percent solution of polyoxyethylene (23) lauryl ether in 25 millimolar aqueous calcium chloride. Also claimed is a biologically pure culture of the microorganism, and a method for the production of the lipase.

Identification, characterization, and method of production of a novel

This invention is directed to a detergent composition comprising the microbial lipase SD2 and dodecylbenzene sulfonate. In the detergent composition, the lipase SD2 is characterized by having (i) optimum pH for activity of about 8.+-.0.5; (ii) an optimum temperature for activity of about 30.degree. to 55.degree. C. and (iii) a molecular weight as measured by gel permeation chromatography of about 8.8.times.10.sup.4.

Cleaning composition comprising microbial lipase SD2 and sodium

A novel lipase from a newly-discovered strain of Pseudomonas alcaligenes microorganism having (i) an optimum pH for activity of about 10.+-.0.5; (ii) an optimum temperature for activity of about 45.degree. to 55.degree. C.; (iii) an optimum pH for stability of about 7.0.+-.0.5; (iv) a molecular weight as measured by gel permeation chromotagraphy of about 8.8.times.10.sup.4 ; and (v) chemical stability for at least 30 days in the presence of the surfactants. Also claimed is a biologically pure culture of the microorganism, and a method for the production of the lipase.

This invention is directed to a detergent composition comprising the microbial lipase SD2, dodecylbenzene sulfonate, and gelatin. In the detergent composition, the lipase SD2 is characterized by having (i) optimum pH for washing activity of about 8.+-.0.5; (ii) an optimum temperature for activity of about 30+ to 55.degree. C. and (iii) a molecular weight as measured by gel permeation chromatography of about 8.8.times.10.sup.4.

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Hamden, CT, United States of America

Paul E Holmes