Gen-probe, Inc.

E.i. Dupont De Nemours and Company

Other

Dupont Safety & Construction, Inc.

Paul Douglas Stull

Marc Spingola

Daniel L Kacian

Nanibhushan Dattagupta

William G Weisburg

Melissa M Cunningham

Yeasing Y Yang

Kostantinos Kourtakis

Michael McClellan Becker

Mark Theodore Aronson

Paul Gregory Bekiarian

Michael P Perry

Joseph D Trentacosta

Gordon Mark Cohen

James M Prober

Kristi K Myers

Kevin Michael Croker

Michael B Damore

Alicia Marie Castagna

This invention relates to a yarn, and a process for making the yarn, the yarn comprising a plurality of spun filaments having a distinct, continuous, uniform-density sheath of a first polymer surrounding a distinct, continuous core of a second polymer; the first polymer further having a thermal decomposition temperature at least 50 degrees Celsius lower than the thermal decomposition temperature of the second polymer.

Yarn from polymers having different decomposition temperatures and process for forming same

This invention relates to the use of certain amine promoters in processes for surface-treating colloidal silica nanoparticles with selected aromatic aminosilanes, aromatic aminoalkylsilanes, and secondary and tertiary aliphatic aminosilanes. This process provides surface-modified nanoparticle colloidal silica without causing the silica nanoparticles to gel, agglomerate, or aggregate.

Amine-accelerated process for the surface treatment of colloidal silica and products thereof

The present invention describes novel oligonucleotides targeted to nucleic acid sequences derived fromorganisms, andorganisms in particular, which are useful for determining the presence oforganisms in a test sample. The oligonucleotides of the present invention include hybridization assay probes, helper probes and amplification primers. The present invention further describes a novel method for obtaining purified ribonucleic acid from viable oocysts.

[object Object]

Compositions and methods for detecting the presence of cryptosporidium organisms in a test sample

Method for obtaining purified RNA from viable oocysts

A microsphere for use in a bioassay comprising a glass core coated with a nanoparticle composite comprising a bioactive probe is provided. The nanoparticle composite coating enhances the density of bioprobe loading on the surface of the microspheres, resulting in enhanced dynamic range and sensitivity in bioassays. The particle may be used in detection systems where resonant light scattering properties of the particle are useful.

Nanoparticle composite-coated glass microspheres for use in bioassays

Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5' exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonu- clease activity.

Isothermal strand displacement nucleic acid amplification

Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5' exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.

Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5&prime; exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.

The present invention features 'promoter-sequestered' oligonucleosides and the use of such oligonucleosides to achieve 'target-triggered' amplification. A promoter-sequestered oligonucleoside contains a contiguous nucleic acid sequence which forms a stem-loop structure in the absence of a target sequence. The stem-loop structure contains a single-stranded loop region and a double-stranded stem region. The single-stranded loop contains all, or a portion of, an RNA polymerase promoter sequence. The stem is produced from two substantially complementary nucleic acid sequences able to form an intramolecular hybrid. The secondary structure of the stem decreases the accessibility of the loop promoter sequence to form a functional double-stranded promoter.

Promoter-sequestered oligonucleoside and method of use

Oligonucleotides and methods for the amplification and specific detection of Chlamydia trachomatis. The invention relates to amplification oligonucleotides capable of amplifying Chlamydia trachomatis nucleotide sequences and to probes and helper oligonucleotides for the specific detection of Chlamydia trachomatis nucleic acids. The invention also relates to methods for using the oligonucleotides of the present invention and specific combinations and kits useful for the detection of Chlamydia trachomatis.

Growing community of inventors

Wilmington, DE, United States of America

Paul Douglas Stull