Organogenesis, Inc.

Paul D Kemp

Robert M Carr, Jr

Eugene Bell

John Cavallaro

Jerome Gross

Ginger A Abraham

Ryan D Mercer

Linda Baker

John F Cavallaro

John Gregory Maresh

Lisa M Bryant

John G Maresh

Valerie Mason

Kathleen Regan

David T Kagan

Lisa Falco

David W Donovan

Reconstituted collagen fiber segment compositions and methods of

The invention is directed to collagenous tissues which have been treated to remove non-collagenous components such as cells, cellular debris, and other extracellular matrix components, such as proteoglycans and glycosaminoglycans, normally found in native tissues. Treatment of the tissue with alkali, chelating agents, acids and salts removes non-collagenous components from the collagenous tissue matrix while controlling the amount of swelling and dissolution so that the resultant collagen matrix retains its structural organization, integrity and bioremodelable properties. The process circumvents the need to use detergents and enzymes which detrimentally affect the cell compatibility, strength and bioremodelability of the collagen matrix. The collagenous tissue matrix is used for implantation, repair, or use in a mammalian host.

Chemical treatment, without detergents or enzymes, of tissue to form an

Tissue equivalents are produced by applying a solution of collagen without cells to a permeable membrane, gelling to produce a collagen gel on the membrane, applying a mixture of collagen and a contractile agent to the collagen gel, gelling the mixture and allowing the resultant gel to undergo radial contraction which is controlled by the collagen gel on the membrane. A suitable contractile agent is fibroblast cells. A skin tissue equivalent is produced by disposing human epidermal cells on the contracted collagen gel and allowing epidermalization to occur. A nutrient medium can be supplied to the cells. An absorbent member may be disposed adjacent the permeable membrane opposite the collagen gel on the membrane. The permeable membrane can be the bottom of an inner well disposed in an outer well for containing a nutrient medium in contact with the membrane. The nutrient medium may be contained in the outer well in an agarose gel in contact with the membrane.

Preparation of tissue equivalents by contraction of a collagen gel

This invention is directed to the sterilization of collagen and collagenous tissue which is to be used for implantation, repair, or use in a mammalian host in the emerging field of tissue engineering. The sterilants are low concentration peracetic acid solutions in either neutral or high ionic strength that prevent or minimizes the swelling of collagen or collagenous tissue so that the sterilized tissue retains its structural integrity and bioremodelable properties.

Peracetic acid sterilization of collagen or collagenous tissue

The present invention provides improved collagen threads and methods of making and using such threads, including the production of yarn, as well as braided, knitted, and woven articles of manufacture, comprising such threads.

Collagen threads

The present invention provides collagen constructs and methods of making and using such constructs. The present invention also provides tissue equivalents having improved characteristics and methods of making and using such tissue equivalents. This invention also provides methods of producing highly concentrated solutions of collagen.

Collagen constructs

This invention relates to collagen compositions and methods of preparing such compositions. This invention also relates to a novel source of collagen, the common digital extensor tendon.

Collagen compositions and methods for preparation thereof

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Cambridge, MA, United States of America

Paul D Kemp