Archer Daniels Midland Company

Iowa State University Research Foundation Inc

Massachusetts Institute of Technology

Other

Paul D Hanke

Thomas P Binder

Aragula Gururaj Rao

Holly J Walsh

Yasufumi Yamamoto

Lhing-Yew Li-D'Elia

Anthony J Sinskey

Laura B Willis

Hungming J Liaw

P John Rayapati

Corey M Crafton

Leif Solheim

Jill S Bradshaw

Richard D Dancey

Stacia L Swisher

Stephane Guillouet

Wuli Bao

Thomas W McMullin

Mark S Dunkak

Ming-Der Wang

Michael R O'Donohue

Described herein are small peptide domains and consensus sequences that bind small target molecules of industrial importance, e.g., metals such as nickel, &#x3b2; carotene, and isoflavones such as genistein. Also described are fusion proteins containing such binding domains fused to proteins or to peptide domains like GST or CBD that bind other ligands and can be used to immobilize the target binding domain on a support. One class of fusion proteins that is useful in industrial settings are fusions that contain concatemers of target binding domains, which increases the binding equivalents per molecule.

Peptide domains that bind small molecules

Peptide domains that bind small molecules of industrial significance

A method of converting hydroxymethylfurfural and is derivative species into hydroxymethylfurfural oxidation products is disclosed. The method includes contacting the hydroxymethylfurfural species in a mixture with an enzyme that oxidizes the hydroxymethylfurfural species while controlling hydrogen peroxide in the mixture. In one exemplary embodiment the enzyme is chloroperoxidase and the hydrogen peroxide is metered into the mixture to predominantly and selectively make at least one of formylfuran carboxylic acid or furan dicarboxylic acid. In another embodiment the enzyme is aryl alcohol oxidase and catalase is included in the mixture to remove unwanted hydrogen peroxide by product and the reaction predominantly makes at least one of dimethylfuran or formylfuran carboxylic acid. When the predominant product is a carboxylic acid or furan dicarboxylic acid, it can be recovered in substantially pure form by acid precipitation.

Enzymatic oxidation of hydroxymethylfurfural

The invention provides methods to increase the production of an amino acid fromspecies by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome. The invention also provides novel processes for the production of an amino acid by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome and/or by increasing promoter strength. In a preferred embodiment, the invention provides processes to increase the production of L-lysine inby way of the amplification of L-lysine biosynthetic pathway genes in a host cell chromosome. The invention also provides novel isolated nucleic acid molecules for L-lysine biosynthetic pathway genes of

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Methods and compositions for increased production of amino acids fromusing sucrose as a carbon source are described. In one aspect, increased production of L-lysine fromis accomplished by using a strain having a mutation in the ptsF gene encoding fructose-PTS enzyme that attenuates or blocks fructose import into the cell when such strain is grown on media containing sucrose as a carbon source and production is increased by providing glucose isomerase in the fermentation media. The glucose isomerase may be exogenously added or expressed in the strain and exported into the media. In certain embodiments the media also contain an invertase. In another aspect increased production of L-lysine is accomplished by making astrain having the ptsF mutation and a second mutation in a fructose exporter function. The dual mutation retains imported fructose in the cell. In certain embodiments, the strain also overexpresses at least one of a glucose isomerase and glucokinase activity in the cell to drive imported fructose toward the pentose phosphate pathway to increase L-lysine production.

The invention provides methods to increase the production of an amino acid fromspecies by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome. Amplification may be by integration of one or more copies of a gene or genes into a host cell chromosome. One gene that may be incorporated is the gene ORF2, which encodes an unnamed hypothetical protein and which may be obtained from. The invention also provides novel isolated nucleic acid molecules for L-lysine biosynthetic pathway genes of

The present invention relates to a mutated pyruvate carboxylase gene from. The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene inwith this feedback-resistant pyruvate carboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.

Feedback-resistant pyruvate carboxylase gene from corynebacterium

Methods for cell-free production of glucosamine from starch, maltodextrin or glycogen or from fructose and a source of amino groups are disclosed. Also disclosed are cellular extracts comprising glucosamine-6-phosphate synthase activity, as well as a cellular extract comprising glucosamine-6-phosphate deaminase.

Cell-free production of glucosamine

The invention provides methods to increase the production of an amino acid from Corynebacterium species by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome. In a preferred embodiment, the invention provides methods to increase the production of L-lysine inby way of the amplification of L-lysine biosynthetic pathway genes in a host cell chromosome. The invention also provides novel processes for the production of an amino acid by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome and/or by increasing promoter strength. In a preferred embodiment, the invention provides processes to increase the production of L-lysine inby way of the amplification of L-lysine biosynthetic pathway genes in a host cell chromosome. The invention also provides novel isolated nucleic acid molecules for L-lysine biosynthetic pathway genes ofsuch as a naturally occurring, feedback-sensitive form of aspartokinase (ask) resulting from a threonine to isoleucine mutation at amino acid residuein the ask gene of ATCC 21529, aspartate-semialdehyde dehydrogenase (asd), dihydrodipicolinate synthase (dapA), dihydrodipicolinate reductase (dapB), diaminopimelate dehydrogenase (ddh), and diaminopimelate decarboxylase (lysA).

Increased lysine production by gene amplification using coryneform bacteria

The present invention relates, in general, to a method of producing L-amino acids comprising culturing altered bacterial cells having increased amounts of NADPH as compared to unaltered bacterial cells whereby L-amino acids yields from said altered bacterial cells are greater than yields from unaltered bacterial cells. The invention also relates to a gene encoding phosphoglucoisomerase.

Methods for producing L-amino acids using a corynebacterium glutamicum with a disrupted pgi gene

Gene encoding phosphoglucoisomerase

An improved recombinant DNA plasmid composed of a DNA vector and other DNA fragments containing the tryptophan operon from E. coli, the aroG gene from E. coli and the serA gene from E. coli is shown. The plasmid is transformed into a microorganism belonging to Escherichia coli, and the microorganisms are cultured in a medium and the L-tryptophan accumulated in the culture is recovered.

Growing community of inventors

Urbana, IL, United States of America

Paul D Hanke