Response Biomedical Corporation

Blood Cell Storage, Inc.

Northwest Biotherapeutics, Inc.

Paul C Harris

Michael W Reed

Steven J Geelhood

Whalley K Fong

Oliver Z Nanassy

Perry Hargrave

Lynn M Barker

Brian G Richards

Randy D Pfalzgraf

Alton L Boynton

Marnix L Bosch

Lynn P Cloney

Patricia A Lodge

Allen Turner

Steven J Monahan

Donald Elliott Brooks

Jonathan G Tippett

Zongcen Charles Xie

Andrew D Olal

System and method for detecting and measuring chemical perturbations in a sample. The system and method are useful for non-invasive pH monitoring of blood or blood products sealed in storage bags.

Fluorescent detector systems for the detection of chemical perturbations in sterile storage devices

The present invention provides tangential flow filtration devices and methods for enriching a heterogenous mixture of blood constituents for leukocytes by removal of non-leukocyte blood constituents. In one particular embodiment the device can provide a composition enriched in monocytes.

Tangential flow filtration devices and methods for leukocyte enrichment

Methods for measuring the amount of two or more analytes of interest in a fluid sample, and kits useful in the methods, are disclosed. The methods involve determining a ratio of a detected amount of a single analyte of interest, to the sum of a detected amount of each of the analytes of interest plus a detected amount of a control, wherein the amount of each analyte of interest is directly or inversely related to the ratio for each analyte of interest.

Multiple analyte immunoassay

Methods of increasing specific binding, decreasing non-specific binding, and reducing false-positive interaction in solid phase assays for influenza are disclosed. In the methods, the solid phase apparatus (lateral flow solid phase apparatus or capillary flow solid phase apparatus) is subjected to elevated heat subsequent to application of a test sample to the solid phase apparatus.

Heated assays for influenza

Methods for quantitatively measuring the amount of an analyte of interest in a fluid sample, and kits useful in the methods, are disclosed. The methods comprise sandwich assays, and utilize an internal calibration reaction that closely mimics the reaction of test particles by the use of a two-step reaction.

Immunoassay employing two-step internal calibration reaction

Fluorescent pH detector and methods for measuring pH using the fluorescent pH detector.

Fluorescent pH detector system and related methods

Methods for measuring the relative amount of two or more analytes of interest in a fluid sample, as well as kits useful in the methods, are disclosed. The methods involve assays that utilize a solid phase apparatus with a membrane having an application point and at least two sample capture zones having sample capture reagents; analyte binding particles or analyte coated particles; and assessment of a ratio of such particles arrested in capture zones, wherein the ratio is equal to, or inversely equal to, the relative amounts of the analytes of interest in the fluid sample.

Comparative multiple analyte assay

The present invention provides tangential flow filtration devices and methods for enriching a heterogeneous mixture of blood constituents for leukocytes by removal of non-leukocyte blood constituents. In one particular embodiment the device can provide a composition enriched in monocytes. One embodiment includes a remover unit () having a crossflow chamber () separated by a microporous filter () from a filtrate chamber (), the remover unit () also having a tangential flow inlet (), a fluid outlet () for a fluid enriched in leukocytes and a filtrate outlet ().

A sample testing apparatus () for use in measuring an analyte in a sample () is disclosed. The apparatus includes a sample well () for receiving the sample. A solid analyte binding agent () is disposed in the sample well. The apparatus further includes a mixing device () for mixing the sample with the solid analyte binding agent and a valve (). The valve is configurable between a closed position for impeding flow of the sample from the sample well and an open position for releasing the sample from the sample well. The apparatus additionally includes a membrane () for receiving the sample once released from the sample well, the membrane having a capture reagent () adapted to interact with at least one of the analyte, the analyte binding agent, or a complex thereof, to aid in determining the amount of the analyte in the sample.

Method and apparatus of quantitative assays

Methods for quantitatively measuring the amount of an analyte of interest in a fluid sample are disclosed. The methods involve providing a membrane having an application point, a detection zone, and a contact region, where the contact region is between the application point and the detection zone, and has test particles and internal control particles imbedded within it; contacting the application point with the fluid sample; maintaining the membrane under conditions sufficient to allow fluid to transport analyte by capillary action to the contact region, where the analyte binds to the test particles; further maintaining the membrane under conditions sufficient to allow fluid to transport analyte-bound test particles and internal control particles to the detection zone, where they interact with a detection reagent; and detecting the amount of test particles and the amount of internal control particles in the detection zone. The amount of analyte in the fluid sample is related to a corrected test particle amount, which can be determined, for example, as the difference between the amount of test particles and the amount of internal control particles in the detection zone. Alternatively, the fluid sample can be contacted with the detection zone of the apparatus, and the test particles are mobilized by addition of fluid to the application point. Other methods involve providing a solid phase reactant for an enzyme-linked immunosorbent assay, and contacting the solid phase reactant with a solution containing an initial detection antibody and an internal control antibody. The amount of analyte in the fluid sample is related to a corrected amount of initial detection antibody, which can be determined, for example, as the difference between the amount of initial detection antibody and the amount of internal control antibody on the solid-phase reactant.

Growing community of inventors

Bothell, WA, United States of America

Paul C Harris