University of California

Paul A Feldstein

Joshua Hihath

Maria Louise Marco

Erkin Seker

James E Lincoln

Bryce William Falk

Andre Knoesen

Yuanhui Li

Zimple Matharu

Pallavi Daggumati

Juan Artes Vivancos

Provided are constructs and methods useful for the screening and identification of polynucleotide sequences of interest, in particular promoters, RNA stability modifying sequences and transcriptional modifying sequences.

Compositions and methods for identifying polynucleotides of interest

A biosensor platform apparatus and method are provided that can detect, purify and identify nucleic acid (DNA and RNA) biomarkers in complex biological fluids. The methods use a two-stage molecular based approach. The first stage screens for specific nucleic acid-based biomarkers in complex biological fluids by electrochemical detection of DNA:RNA hybridization and facilitates the removal of remaining complex media constituents. The first stage utilizes probes within a tunable nanoporous electrode. The second stage identifies the purified specific hybrids by single-molecule conductance measurements via break junction scanning. Identification can be assisted with a library of conductance measurements. The methods can provide strain level information that can be used for identifying anti-microbial resistance in detected pathogens. Collection of RNA targets allows for biomarker detection and identification without the need for amplification and can provide information about the viability of the sample organism.

Integrated electrochemical detection and purification of nucleic acid biomarkers

Provided are constructs and methods for RNA promoter identification.

Methods for RNA promoter identification

A technique that uses nanotechnology to electrically detect and identify RNA sequences without the need for using enzymatic amplification methods or fluorescent labels. The technique may be scaled into large multiplexed arrays for high-throughput and rapid screening. The technique is further able to differentiate closely related variants of a given bacterial or viral species or strain. This technique addresses the need for a quick, efficient, and inexpensive bacterial and viral detection and identification system.

RNA-based, amplification-free, organism identification using nano-enabled electronic detection

Growing community of inventors

San Francisco, CA, United States of America

Paul A Feldstein