Other

University of Kentucky Research Foundation

Patrizia Pasini

Sapna Kamlakar Deo

Anjali Kumari Struss

Harohalli Shashidhar

Deborah R Auer Flomenhoft

Nilesh Raut

Amol Date

The presently-disclosed subject matter provides systems, methods, and kits for diagnosing and/or monitoring a bacteria-related condition of interest in a subject by providing a cell sensing system, each system containing a reporter molecule capable of detecting binding of a quorum sensing molecule and capable of generating a detectable signal.

Systems and methods for diagnosis and monitoring of bacteria-related conditions

The presently-disclosed subject matter is directed to biosensors comprising spore-forming bacterial cells and/or spores generated therefrom, a recognition unit within each spore-forming cell for binding an analyte of interest, and a reporter molecule within each spore-forming cell for detecting binding of the analyte of interest, wherein the reporter molecule generates a detectable signal upon binding of the analyte by the recognition element. The presently-disclosed subject matter further provides methods of using the biosensors and systems and kits including the biosensors.

Spores for the stabilization and on-site application of bacterial whole-cell biosensing systems

Growing community of inventors

Lexington, KY, United States of America

Patrizia Pasini