University of Iowa Research Foundation

University of California

Iowa State University Research Foundation, Inc.

Boyce Thompson Institute for Plant Research

Patrick Sinn

Paul B McCray, Jr

Mark Aaron Behlke

Beverly L Davidson

Hong Peng Jia

Paul Mccray

Maureen D Donovan

Anthony J Fischer

Daniel F Voytas

Gary W Blissard

Hung Fan

Junbiao Dai

A method of co-expressing a portion of the VSV G protein gene or a truncated 'stem' portion with GP64 and a retrovirus increases the titer of retroviral vectors. A truncated VSV G protein, preferably comprised of a small segment from the C-terminal portion of the ectodomain plus the transmembrane (TM) and cytoplasmic tail (CTD) domains of VSV G, co-expressed with retroviral vectors, enhances the production titers of the retroviral vectors. A preferred embodiment uses a VSV G construct that includes an N-terminal c-Myc epitope plus 42 amino acids from the C-terminal portion of the ectodomain, 20 amino acids from the predicted TM domain, and 29 amino acids from the predicted CTD of the VSV G protein.

Baculoviruses with enhanced virion production and a method for the production of baculoviruses

The present invention is directed to small interfering RNA molecules targeted against a gene of interest in respiratory epithelial cells, and methods of using these RNA molecules.

RNA interference in respiratory epithelial cells

The present invention concerns the methods and compositions related to pseudotyped viral vectors. Embodiments of the invention include pseudotyping expression cassettes that include nucleic acid elements for enhancing the titer of pseudotyped viral particles. Embodiments of the invention include novel methods and compositions related to making high titer pseudotyped retroviral vector compositions. A heterologous envelope glycoprotein is typically incorporated into the virus during the budding or virus production process. Certain embodiments of the invention include pseudotyped retroviral vectors comprising a heterologous envelope glycoprotein derived from a Jaagsiekte sheep retrovirus (JSRV env). Pseudotyped viruses or viral particles may have a modified host range that is influenced by the properties of the heterologous envelope glycoprotein. Thus, embodiments of the invention include improved methods and compositions related to pseudotyped viruses suitable for ex vivo and in vivo methods including gene transfer and other therapeutic and experimental methods.

Methods and compositions related to high-titer pseudotyped retroviruses

Embodiments of the invention include compositions comprising and methods utilizing a retroviral integrase complex comprising a recombinant integrase having a domain comprising a non-native protein binding site, and a DNA binding protein comprising a DNA binding domain and a peptide binding domain that binds the non-native protein binding site of the recombinant integrase.

Compositions and methods related to modified retroviral vectors for restricted, site specific integration

RNA interference in respiratory epitheial cells

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Iowa City, IA, United States of America

Patrick Sinn