Iowa State University Research Foundation Inc

The United States of America As Represented by the Secretary of

Data2bio

Dryland Genetics, Inc.

Other

Patrick S Schnable

Basil J Nikolau

Eve Syrkin Wurtele

Yan Fu

Feng Liu

Tsui-Jung Wen

David J Oliver

Isabelle Lutziger

Wei Wu

Sudhansu Dash

Roger P Wise

Beth Fatland

James C Schnable

Santosh Rajput

Jinshan Ke

Robert Behal

Sanzhen Liu

Jerry L Johnson

Carolyn C Allred

Donald S Robertson

Ruth A Swanson-Wagner

Dan Nettleton

Joel D Hansen

Yiji Xia

Xiaojie Xu

Jinliang Yang

Several Proso cultivars are disclosed having improved yields. The technology relates to the seeds, plants, and plant parts of Proso cultivars; and further provides methods for producing progeny of Proso cultivars. The technology also relates to Proso cultivars or breeding cultivars, and plant parts derived from Proso cultivars; and to methods for producing other Proso cultivars, lines, or plant parts derived from Proso cultivars, and to the Proso plants, varieties, and their parts derived from use of those methods. The technology further relates to hybrid Proso seeds, plants, and plant parts produced by crossing improved-yield Proso cultivars with another Proso cultivar.

Higher-yielding proso

Provided herein is technology relating to genotyping and particularly, but not exclusively, to methods for genotyping one or more organisms by genome sequencing.

Genotyping by next-generation sequencing

The present invention relates nucleic acid molecules that are modulated (e.g., upregulated) by nitrogen in corn, to proteins or polypeptides encoded by these nucleic acid molecules, and promoters of these nucleic acid molecules. The present invention relates to a nucleic acid construct having a nucleic acid molecule that is modulated by nitrogen in corn, as well as to expression systems, host cells, plants, and plant seeds having the nucleic acid construct. The present invention also relates to a method of expressing the nucleic acid molecule that is modulated by nitrogen in a plant by growing a transgenic plant or a plant grown from a transgenic seed transformed with the construct. The present invention further relates to an isolated DNA promoter that can be used to direct nitrogen-regulated expression of an isolated nucleic acid in plants.

Plant genes involved in nitrate uptake and metabolism

The present invention relates to a method for determining an ear productivity trait in maize, which involves analyzing genomic DNA from a maize plant, germplasm, pollen, or seed for the presence of a molecular marker linked to a QTL associated with an ear productivity trait in maize. Also disclosed are methods of selecting a maize plant with a desired ear productivity trait; reliably and predictably introgressing an improved ear productivity trait into a maize line; and producing a maize line having a desired ear productivity trait. A kit for selecting a maize plant by marker assisted selection of a QTL associated with a desired ear productivity trait; an isolated nucleic acid comprising a QTL associated with an ear productivity trait in maize; and a transgenic plant comprising a recombinant nucleic acid genetically linked to a locus in maize, are also disclosed.

QTL regulating ear productivity traits in maize

The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL),pyruvate decarboxylase (PDC), andaldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1&#x3b1; subunit of pPDH, the E1&#x3b2; subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1&#x3b1; pPDH, E1&#x3b2; pPDH, E2 pPDH, mtPDH or ALDH.

Materials and methods for the alteration of enzyme and acetyl CoA levels in plants

Compositions and methods for identifying nucleotide fragments that contain an open reading frame are provided. Compositions comprise a nucleotide sequence that encodes, in each of the three possible reading frames, an ATG start codon and a histidine tag, and vectors comprising such a nucleotide sequence. The vectors may be provided with cloning sites for insertion of nucleotide sequences of interest 5' or 3&#x2032; to the 3-frame His-tag DNA sequence. Vectors also are provided with cloning sites for inserting nucleotide sequences of interest 3&#x2032; of the ATG start codon and 5&#x2032; of the 3-frame His-tag DNA sequence.

Method of identifying an open reading frame using a nucleic acid molecule encoding multiple start codons and histidine tags

The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1 subunit of pPDH, the E1 subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyurvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1 pPDH, E1 pPDH, E2 pPDH, mtPDH or ALDH.

Compositions and methods for identifying nucleotide fragments that contain an open reading frame are provided. Compositions comprise a nucleotide sequence that encodes, in each of the three possible reading frames, an ATG start codon and a histidine tag, and vectors comprising such a nucleotide sequence. The vectors may be provided with cloning sites for insertion of nucleotide sequences of interest 5&prime; or 3&prime; to the 3-frame His-tag DNA sequence. Vectors also are provided with cloning sites for inserting nucleotide sequences of interest 3&prime; of the ATG start codon and 5&prime; of the 3-frame His-tag DNA sequence.

Nucleic acid molecules encoding multiple start codons and histidine tags

Compositions and methods for identifying nucleotide fragments that contain an open reading frame are provided. Compositions comprise a nucleotide sequence that encodes histidine tags in each of the three possible reading frames and vectors comprising such a nucleotide sequence. The vectors may be provided with cloning sites for insertion of nucleotide sequences 5&prime; or 3&prime; to the 3-frame His-tag DNA sequence.

Nucleic acid molecules encoding histidine tags in three reading frames

The present invention relates to cuticular lipid genes, their gene products, and methods of use thereof, for the generation of new plant varieties having novel environmental, disease and pest resistance. In particular, certain isolated or enriched nucleic acids specifying cuticular lipid genes, or portions thereof, are disclosed.

Isolation and use of cuticular lipid genes

The present invention relates to the field of producing hybrid seeds, and specifically relates to the application of certain cloned nuclear fertility restorer genes, which restore male fertility to cytoplasmic male-sterile plants. In particular, the present invention relates to an enriched or substantially isolated nucleic acid sequence comprising a nucleotide sequence that hybridizes at least under moderately stringent conditions to SEQ ID NOS: 1, 3 or 4 and restores male fertility to a cytoplasmic male-sterile plant; a vector comprising such a sequence; an organism, virus, seed, plant cell, and plant comprising such a vector; methods for the production of hybrid seed; a method of producing a variant of a cytoplasmic male-sterile plant, and a method of suppressing cytoplasmic male sterility, all of which make use of the aforementioned nucleic acid sequences.

Growing community of inventors

Ames, IA, United States of America

Patrick S Schnable