Oxtox Limited

Shell Internationale Research Maatschappij, B.v.

Patrick Robinson Huddleston

Craig Edward Banks

Dimitrios Konstantinos Kampouris

John M Barker

Rashid Olukayoee Kadara

The invention provides an electrochemical assay for a phenol analyte in a body fluid sample wherein said sample, or fluid therefrom, is contacted with the working electrode of an electrode assembly comprising a working electrode, a counter-electrode, a voltage supply to said working and counter-electrodes and a current meter for determining the current between said working and counter-electrodes, and wherein a first compound capable of reversible oxidation and reduction is disposed at said working electrode, said first compound being capable in either an oxidized or a reduced form of binding to said phenol analyte, characterized in that said first compound in its oxidized or reduced form comprises a group of structure I R1-NH&#x2014;C*&#x2014;(C*&#x2014;C*)&#x2014;C*-QR' (I) (where n is 0 or 1; Q is 0, S, NH or NR&#x2032;; C*&#x2014;(C*&#x2014;C*)&#x2014;C* is a two or four carbon string in a conjugated delocalised electron system optionally substituted by a group comprising R; R is an electron-donating or withdrawing substituent; and R&#x2032; is H or a group R, at least one group R&#x2032; being a group R).

Electrochemical assay

The invention provides a method of assaying for tetrahydrocannabinol in a body fluid. The method includes contacting a sample of body fluid with an imine capable of reacting with tetrahydrocannabinol to yield a quinone imine, and detecting the formation of a quinone imine, where the sample is contacted with the reagent compound at a pH of at least 10.5.

Methods of assaying for tetrahydrocannabinol

The invention provides an assay method for the detection of a primary amine analyze in an aqueous body fluid, which method comprises contacting a sample of said body fluid at a pH below 9.5 with a thiol and an unsaturated cis-dialdehyde and testing for the presence of a pyrrole reaction product.

Assay method for detecting primary amines

A compound of the general formula ##STR1## in which X represents a chlorine or bromine atom, R.sup.1 represents a hydrogen atom, a C(1-6) alkyl group, a benzyl group or a phenyl group optionally substituted by a C(1-6) alkyl group, and R.sup.2 represents the group CN or the group COOR.sup.3 in which R.sup.3 represents a C(1-6) alkyl group, a process for the preparation of a compound of formula (I), and the use of a compound of formula (I) in the preparation of a compound of the general formula. ##STR2## in which R.sup.1 is as defined above and R.sup.4 represents an amino group or a hydroxyl group.

Thiapentanamide derivatives

A process for the preparation of 3-aminothiophenes or acid-addition salts thereof, which comprises reacting the corresponding 3-oxotetrahydrothiophenes with an acid-addition salt of hydroxylamine in the presence of a polar inert solvent and in the absence of a base at a temperature in the range of from 0.degree. to 200.degree. C.

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Nottingham, United Kingdom

Patrick Robinson Huddleston