Cold Spring Harbor Laboratory

Merck + Co., Inc.

Princeton University

Patrick Paddison

Gregory J Hannon

Douglas S Conklin

Scott Hammond

Emily Bernstein

Amy Caudy

Stephen H Friend

Michele A Cleary

Ernest M Coffey

Ihor R Lemischka

Christoph Schaniel

Kristopher A Killian

Kristopher A Kilian

Feng Li

Xenla Schafer

The present invention provides methods for attenuating gene expression in a cell, especially in a mammalian cell, using gene-targeted double stranded RNA (dsRNA), such as a hairpin RNA. The dsRNA contains a nucleotide sequence that hybridizes under physiologic conditions of the cell to the nucleotide sequence of at least a portion of the gene to be inhibited (the 'target' gene).

Methods and compositions for RNA interference

The present invention provides methods of making a population of nucleic acid molecules, wherein each nucleic acid molecule comprises a predetermined nucleic acid sequence, each of said methods comprising the steps of: (a) synthesizing, on a substrate, a population of nucleic acid molecules wherein: i) each synthesized nucleic acid molecule comprises a predetermined nucleic acid sequence; and ii) each synthesized nucleic acid molecule is localized to a defined area of said substrate; (b) harvesting said population of synthesized nucleic acid molecules from said substrate to yield harvested nucleic acid molecules; and (c) introducing said harvested nucleic acid molecules into vector molecules.

Method for making populations of defined nucleic acid molecules

The present invention provides methods for attenuating gene expression in a cell using gene-targeted double stranded RNA (dsRNA). The dsRNA contains a nucleotide sequence that hybridizes under physiologic conditions of the cell to the nucleotide sequence of at least a portion of the gene to be inhibited (the 'target' gene).

The present invention relates to methods and compositions for assaying embryonic stem cell maintenance. In particular, the present invention provides reporter constructs for stem cell pluripotency and differentiation and cells and organisms containing such constructs.

Embryonic stem cell self maintenance and renewal reporter

Growing community of inventors

Seattle, WA, United States of America

Patrick Paddison