Centre National De La Recherche Scientifique

Université D'orleans

Université D'evry-Val-D'essonne

Université De Bretagne Occidentale

Patrick Midoux

Mathieu Mevel

Chantal Pichon

Jean-Marc Malinge

Thomas Thibault

Hervé Cheradame

Mathieu Sassatelli

Philippe Guegan

Lucie Pigeon

Rachid A Rahmouni

Harivony Chantal Pichon-Rabenandrasana

Jean-Claude Clement

A Rachid Rahmouni

Harivony Pichon

The application relates to the production of RNA of interest, more specifically of messenger RNA of interest or of long non-coding RNA of interest, by yeasts with recombinant pseudo-viral particles. The recombinant yeasts have been genetically modified in order to produce the RNA of interest in the form of a complex, particularly in the form of recombinant pseudo-viral particles. These recombinant pseudo-viral particles are produced from certain elements of yeast Ty retrotransposon, but do not retrotranscribe the RNA that they contain. Thus, the application relates to the components that are thus capable of being implemented or produced, and particularly to the nucleic acid constructs, kits, bacteria cells, yeast cells, culture or transfection media containing them, as well as to a method for producing a pharmaceutical composition, particularly for medical applications, more specifically for vaccines, anti-tumour and pro-regenerative applications.

Production of RNA by yeasts with recombinant pseudo-viral particles

A method for the in vitro production of DNA minicircles includes steps of: a) providing nicked double-stranded oligodeoxynucleotides bunt-ended substrates having at least one phosphorylated 5' end, b) performing a ligase-mediated circularization on a reaction mixture including the nicked double-stranded oligodeoxynucleotides substrates and a DNA bending protein, and c) obtaining DNA minicircles.

In vitro production of DNA minicircles comprising less than 250 base pairs

The invention relates to a static copolymer of linear polyethylenimine that comprises two monomer units of the following formulas I and II, in which Ris a hydrogen atom or a C-Calkyl radical, Ris a hydrogen atom, a C-Calkyl, aryl or aralkyl group, wherein the alkyl group is a C-Cone, n is a number between 1 and 99% of the total monomers, m is a number between 1 and 99% of the total monomers, and advantageously m+n=100%. The invention also relates to complexes of this copolymer with a nucleic acid, and to the use thereof in in vivo, in vitro or ex vivo gene transfection. 

Polymer derived from linear polyethylenimine for gene transfer

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Saint Denis de l'Hotel, France

Patrick Midoux