Henkel Ag & Company, Kgaa

Ab Enzymes Gmbh

B.r.a.i.n. Biotechnology Research and Information Network Ag

Degussa Aktiengesellschaft

Ab Enzymes Oy

Patrick Lorenz

Susanne Wieland

Karl-Heinz Maurer

Beatrix Kottwitz

Jürgen Eck

Frank Niehaus

Nina Mussmann

Khanh Quoc Nguyen

Volker Marschner

Kornelia Titze

Tatiana Schwarz

Silvia Paladino

Terhi Puranen

Leena Valtakari

Kari Juntunen

Pentti Ojapalo

Daniela Herbst

Christian Degering

Roland Breves

Ulrich Schwaneberg

Holger Zinke

Oliver May

Taija Leinonen

Harald Trauthwein

Harald Gröger

Renate Schulze

Michael Seefried

Christian P Kubicek

Felix Jakob

Bernhard Seiboth

Shohana Islam

Arnulf Kletzin

Jari Vehmaanperae

Kristiina Jaervinen

Daniela Dollak

Christa Schleper

Ruth Schwerdtfeger

Achim Quaiser

Rita Linke

Torsten Ochsenreiter

Alexander H Treusch

Thomas Haarmann

Quoc Khanh Nguyen

Wolfgang Zimmermann

Ren Wei

Jasmin Eidner

Kristin Ruebsam

Lina Apitius

Nina Mußmann

Martina Schreiter

Kristiina Järvinen

Michael Rachinger

Jari VehmaanperÄ

This invention relates to novel detergent compositions comprising bacterial mannanase enzymes. The detergent compositions comprising bacterial mannanases are useful in laundry and cleaning applications wherein degradation or modification of mannan is desired. The invention also relates to the use of said detergent compositions in laundry and cleaning applications as well as a method for degrading mannan.

Detergent compositions comprising bacterial mannanases

An agent, such as a detergent or cleaning agent, may include a polyesterase. A method for cleaning textiles may include applying the agent to a textile for removing soiling. The polyesterase may also help to reduce pilling effects in the agent.

Agent containing a recombinant polyesterase

The invention relates to a process for identifying gene(s)/genetic element(s) associated with mating impairment in strains ofQM6a or strains derived thereof comprising the steps of a) providing a first strain being aQM6a strain having a MAT1-2 locus or a strain derived thereof, b) sexually crossing said strain with a second strain being a mating competent strain of a() strain having a complementary locus, i.e. the MAT1-1 locus, c) repeatedly back-crossing the MAT1-1 progenies from the crossing of b) or the back-crossing thereof with the first strain of a), until a strain is obtained that is substantially identical to the firstQM6a strain or a strain derived thereof, but carries the MAT1-1 locus and is mating competent for crossing withQM6a or any of its MAT1-2 progeny, d) selecting the progeny from step c) that is mating competent for crossing with a() having a MAT1-2 locus, and e) identifying the gene(s)/genetic element(s) associated with mating impairment by comparing the genome of the progenies selected in step d) with the genome sequences of the first strain of a) whereby said gene(s)/genetic element(s) may be fully or partially missing or existing in a mutated form or in a form having deletions or insertions in the first strain thus being a gene or a genetic element directly or indirectly associated with mating impairment in strains ofQM6a or a strain derived thereof as well as to a process for correcting the mating impairment of aQM6a strain or a strain derived thereof having a MAT1-1 locus and that is not competent to mate with aQM6a strain having a MAT1-2 locus or a strain derived thereof, wherein one or more mutated or fully or partially missing gene(s) and/or genetic element(s) identified as above is/are replaced by or complemented with the corresponding functional gene(s) and/or genetic element(s). Moreover, the invention relates to the use of a thus obtained fungal strain of the genusin industrial breeding and production of a product of interest. Moreover, the invention relates to the genes associated with mating impairment ofQM6a and strains derived therefrom and to genes essential for mating ofQM6a and strains derived therefrom.

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The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID NO: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for by the plasmid pPL3940-Topo2.5 with the restriction map according to FIG., which is deposited under accession number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) due to the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from, and more preferably from, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: 2 or a sequence derived therefrom, which may be obtained by substitution, addition, deletion of one or more amino acid(s), c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide which is coded for by a nucleic acid sequence which hybridizes under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acid(s), f) allelic variants to amino acid sequences a) to e).

Cloning, expression and use of acid phospholipases

The present application relates to two novel proteases (SEQ ID NO: 4 and 7) which are similar to one another. The DNA of the two proteases, was obtained from soil samples, and C-terminally deleted, producing proteolytically active fragments thereof (SEQ ID NO: 5 and 8). The invention also provides all alkaline proteases similar at least to 90% to SEQ ID NO: 4 or to 87.5% to SEQ ID NO: 7, and those which can be summarized under a consensus sequence (SEQ ID NO: 9) derived from SEQ ID NO: 4 and 7. Furthermore, it relates to all nucleic acids which have a homology of at least 85% identity to the associated nucleic acids (SEQ ID NO: 3 and 6) or the fragments concerned. Furthermore, it defines technical possibilities of use for these proteases and especially describes their use in detergents and cleaners.

Alkaline proteases and detergents and cleaners comprising these alkaline proteases

An alkaline protease, the DNA thereof having been taken from a bottom sample, alkaline proteases that are at least 40% identical, and nucleic acids with an identity at least 50% homologous with the associated nucleic acids. The invention also relates to the fragment of said protease comprising the amino acid positions 108 to 325, in addition to the gene fragment coding therefor, and alkaline proteases that are at least 60% identical or nucleic acids that are at least 70% identical. Furthermore, the invention defines technical possibilities of use for said proteases, especially use thereof in washing and cleaning products.

Alkaline protease and washing and cleaning products containing said novel alkaline protease

The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.

Isolation and cloning of DNA from uncultivated organisms

The invention relates to novel polypeptides which have the biological activity of an NAD- or NADP-dependent alcohol dehydrogenase. The invention furthermore relates to nucleic acids encoding said polypeptides, to nonhuman hosts or host cells and to reaction systems which may be used for preparing desired products. The polypeptides of the invention are preferably used in the preparation, starting from aldehydes or ketones, of primary and enantiomerically pure secondary alcohols which may serve as intermediates for medicaments. Alternatively, the polypeptides of the invention may also be employed in the reverse reaction, i.e. the oxidation of alcohols with the formation of aldehydes or ketones.

Alcohol dehydrogenases

The present application relates to two novel alkaline proteases (SEQ ID NO. 4 and 7) which are similar to one another, whose DNA was obtained from soil samples, and which were C-terminally deleted. The present application also provides proteolytically active fragments thereof (SEQ ID NO. 5 and 8), all alkaline proteases similar at least to 90% to SEQ ID NO. 4 or to 87.5% to SEQ ID NO. 7, and those which can be summarized under a consensus sequence (SEQ ID NO. 9) derived from SEQ ID NO. 4 and 7. Furthermore, the present application relates to all nucleic acids which have a homology of at least 85% identity to the associated nucleic acids (SEQ ID NO. 3 and 6) or the fragments concerned. Furthermore, the present application relates to use for these proteases and especially describes their use in detergents and cleaners.

The invention relates to a new glycosyl hydrolases with an amyloltic activity and nucleic acids coding for said gylcosyl hydrolases, A PCR-based method for identifying and preparing new gylcosyl hydrolases from metagenome DNA and several possible technical uses for such glycosyl hydrolases with an amylolytic activity. Washing and cleaning products containing such enzymes, and methods and possible uses corresponding thereto are particularly interesting.

Glycosyl hydrolases

Growing community of inventors

Lorsch, Germany

Patrick Lorenz