Minnesota Mining and Manufacturing Company

3m Innovative Properties Company

University of Minnesota

Coley Pharmaceutical Group, Inc.

Patrick L Coleman

Jerald K Rasmussen

Dean S Milbrath

Steven M Heilmann

Richard J Rolando

Larry R Krepski

Steven L Kangas

Julie B Stahl

Thomas A Kotnour

Margaret M Walker

Kyle J Lindstrom

Bryon A Merrill

Michael J Rice

Stephen L Crooks

Douglas A Hanggi

Peter W Carr

Eric F Funkenbusch

Martin P Rigney

Wes A Schafer

George W Griesgraber

Christopher Stewart Lyons

David R Gagnon

Kurt J Halverson

William Bedingham

Mark Kevitt Debe

Donald F Hagen

Paul E Hansen

James I Hembre

Raj Rajagopal

Peter D Ludowise

Sanjay L Patil

Robert H Menzies

Gary J Drtina

Bathsheba E Chong Conklin

Howard J Buttery

Anila Prabhu

Oh-Seung Kwon

Isidro Angelo E Zarraga

Barbara C Swenson

Gary J Ortina

Samuel J Gason

Jennifer J Sahlin

John C Campbell

Patrick S Quint

Imidazoquinoline and tetrahydroimidazoquinoline compounds that contain amide functionality at the 1-position are useful as immune response modifiers. The compounds and compositions of the invention can induce the biosynthesis of various cytokines and are useful in the treatment of a variety of conditions including viral diseases and neoplastic diseases.

Amide substituted imidazoquinolines

A device includes a substrate having first and second major surfaces and a hub that defines an axis of rotation for the substrate, and an unvented channel having a plurality of connected compartments. Methods for using devices of the invention are also disclosed.

Sample processing device with unvented channel

A coated laminate having an ionic surface including a shrinkable polymeric film, an ionic coating and, optionally, a hydrogel is disclosed. A method for transferring sample molecules from a matrix to a coated laminate having an ionic surface also is disclosed.

Coated film laminate having an ionic surface

Azlactone-functional supports are used to provide cell selection from a mixture such as bone marrow or peripheral blood having a desired target cell population. An azlactone-functional support is derivatized by covalently coupling to the support a biologically active substance that binds the target cells. A mixture containing the target cells is contacted with the derivatized support to bind the target cells to the biologically active substance, and unbound remaining mixture is removed from the support. Bound cells may be eluted from the support to obtain purified target cells. Biologically active substances include antibodies, lectins, proteins, antigens and avidin. The biologically active substance may directly or indirectly bind cells in the mixture. Indirect binding may be through a second, intermediary biologically active substance that is bifunctional. The azlactone-functional support is provided by incorporating an azlactone moiety into a base polymer support that has been selected by prescreening base polymer supports with a cell mixture to identify a base polymer support having minimal nonspecific binding of cells in the mixture.

Method for cell selection utilizing azlactone-functional supports

A composite article is provided having covalently reactive particles incorporated in a continuous, porous matrix. The reactive particles have surfaces of covalently reactive functional groups capable of directly forming covalent chemical bonds with ligands without need for an intermediate activation step. An adduct composite article is also provided comprising a continuous, porous matrix and derivatized particles dispersed therein. The derivatized particles comprise a direct, covalent reaction product of ligand with the covalently reactive particles. Methods of making and using the composite articles and adduct composite articles are also provided. Preferred covalently reactive functional groups are azlactone-functional groups of the formula: ##STR1## wherein: R.sup.1 and R.sup.2 independently can be an alkyl group having 1 to 14 carbon atoms, a cycloalkyl group having 3 to 14 carbon atoms, an aryl group having 5 to 12 ring atoms, an arenyl group having 6 to 26 carbon and 0 to 3 S, N, and nonperoxidic O heteroatoms, or R.sup.1 and R.sup.2 taken together with the carbon to which they are joined can form a carbocyclic ring containing 4 to 12 ring atoms, and

Covalently reactive particles incorporated in a continous porous matrix

The present invention provides biological assay and adsorption supports comprising an inert substrate supporting a nanostructured surface comprising metal coated, oriented, discrete submicron-size whiskers. Optionally, conformally metal coated whiskers may be partially encapsulated or conformally coated with a second material. The nanostructured surface adsorbs biomolecules rapidly at high levels of tight binding without vortexing or agitation. Advantageously, the bound biomolecules retain their biological activity.

Biological adsorption supports

An azlactone-functional thermoplastic composition is disclosed, The composition is a blend of an azlactone-functional composition and a thermoplastic polymer. A method of making azlactone-functional homopolymers by bulk homopolymerization is disclosed, optionally also concurrent or sequential blending of a thermoplastic polymer. A method of blending azlactone-functional compositions and thermoplastic polymers is disclosed. Molded articles coupled with biologically active substances are claimed.

Azlactone-functional polymer blends, articles produced therefrom and

Graft copolymers comprising a poly-alpha-olefin base polymer selected from the group consisting of polyethylene, polypropylene, polystyrene, and compatible mixtures thereof, having grafted thereto an olefinic monomer. The grafted monomer is present in an amount effective to increase the amount of protein that will bind to the graft copolymer as compared with the base polymer. Also disclosed are polymer/protein compositions comprising a graft copolymer having a protein immobilized on the surface thereof, processes for the preparation of the above-described graft copolymers and compositions, methods of immobilizing proteins, and methods of immunoassay based on such immobilization.

Graft copolymers and graft copolymer/protein compositions

Supports having azlactone-functional surfaces, adduct supports prepared from such azlactone-functional supports, and methods of preparing both are disclosed. Azlactone functionality is introduced to surfaces of a pre-existing support in a manner which retains useful physical and chemical characteristics of the pre-existing support. One method involves exposing surfaces with high energy radiation to generate free radical reaction sites on the surfaces and causing azlactone-functional moieties to react with the free radical reaction sites. Another method involves coating surfaces with azlactone monomers, crosslinking monomers, and optionally co-monomers and polymerizing the monomers to form a polymerized coating of azlactone-functionality on the surfaces. Another method involves dispersion polymerization of azlactone-functional moieties to produce azlactone-functional particles within pores and interstices of a pre-existing support. Adduct supports are formed by coupling nucleophilic reagents, such as biologically active materials, to azlactone-functional moieties of the support.

Supports having azlactone-functional surfaces, adduct supports, and

An azlactone-functional thermoplastic composition is disclosed. The composition is a blend of an azlactone-functional composition and a thermoplastic polymer. A method of making azlactone-functional homopolymers by bulk homopolymerization is disclosed, optionally also concurrent or sequential blending of a thermoplastic polymer. A method of blending azlactone-functional compositions and thermoplastic polymers is disclosed. Molded articles and adduct molded articles formed from the thermoplastic composition are disclosed.

A biomosaic polymer is provided. Biologically active materials are bound at surfaces of such polymers polymerized from emulsions containing hydrophobic polymerizable monomers, such biologically active materials, and surface active agents. The biomosaic polymers may be formed into membranes, films, beads, or other structures for a variety of assays, bioseparations, or catalyzed reactions and other uses. A single step polymerization of the emulsion provides significant retention of the biologically active material bound and congregated at surfaces of the polymer.

Biomosaic polymer obtained by emulsion polymerization of hydrophobic

Azlactone-functional polymer supports are useful reactive supports for the attachment of functional materials to provide novel adduct beads. The adduct beads are useful as complexing agents, catalysts, polymeric reagents, chromatographic supports, and as enzyme- or other biologically active supports. Novel carboxylate-functional polymer beads, are intermediates in the preparation of the azlactone-functional beads. Azlactone-functional supports have units of the formula: ##STR1## wherein R.sup.1 is H or CH.sub.3,

Polymeric supports

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Minneapolis, MN, United States of America

Patrick L Coleman