Agilent Technologies, Inc.

Singular Bio, Inc.

Invitae Corporation

Patrick James Collins

Adrian Nielsen Fehr

Hywel Bowden Jones

Jill Lyndon Herschleb

Karen W Shannon

John Frederick Corson

Peter G Webb

Paul K Wolber

Eric M Leproust

Steven M Lefkowitz

Glenda Choate Delenstarr

Jayati Ghosh

Bill J Peck

Charles David Troup

Xiangyang Zhou

Nelson R Holcomb

Keith C Butler

Sandra L Tang

The invention relates to methods of detecting a genetic variation in a genetic sample from a subject using labeled probes and counting the number of labels in the probes. The invention also relates to manufacturing and using molecular arrays and analytical approaches based on single molecule detection techniques.

Arrays for single molecule detection and use thereof

The invention relates to methods of detecting a genetic variation in a genetic sample from a subject using labeled probes and counting the number of labels in the probes.

Assays for single molecule detection and use thereof

A method and system for quantifying and correcting spatial-intensity trends for each channel of a microarray data set having one or more channels. The method and system of one embodiment of the present invention selects a set of features from each channel of the microarray data set. Based on the selected set of features, a surface is used to determine the intensities for all features in each channel of the microarray data set. Spatial-intensity trends within the microarray data set are quantified, based on the surface to the intensities for each channel of the microarray data set. After the surface has been determined, the spatial-intensity trend can be removed from the microarray data set.

Method and system for quantifying and removing spatial-intensity trends in microarray data

Methods of identifying a sequence of a nucleic acid that is suitable for use as a surface immobilized probe for two or more mRNA transcripts encoded by the same gene are provided. In practicing the subject methods, a consensus region for the two or more transcripts is first identified, and this identified consensus region is then employed to identify the suitable nucleic acid sequence, e.g., by using a probe design protocol. The subject invention also includes algorithms for performing the subject methods recorded on a computer readable medium, as well as computational analysis systems that include the same. Also provided are nucleic acid arrays produced with probes having sequences identified by the subject methods, as well as methods for using the same.

Methods designing multiple mRNA transcript nucleic acid probe sequences for use in nucleic acid arrays

A buffer composition, method and kit for hybridizing microarrays of nucleic acids bound to an adsorbed polymer surface of a siliceous substrate provide an envelope of conditions to hybridize nucleic acid targets, while preserving theintactness of the adsorbed polymer surface of the array. The buffer composition comprises a non-chelating buffering agent, a pH within a range of pH 6.4 and 7.5, a monovalent cation having a monovalent cation concentration that ranges from about 0.01 M to about 2.0 M, and optionally relatively lower concentrations of a chelating agent and an ionic surfactant. The total cation concentration of the buffer composition ranges from about 0.02 M to about 2.0 M. The method comprises incubating the targets with the microarray in the buffer composition at a temperature between about 55&deg; C. and 70&deg; C.

Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces

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Patrick James Collins