University Technologies International, Inc.

Patrick J Farrell

Kostas Iatrou

Leo A Behie

Yoshifumi Hashimoto

An baculovirus artificial chromosome is disclosed which has the lef-8 gene inactivated. The baculovirus artificial chromosome allows the cloning and expression of heterologous genes in insect and mammalian cells without killing the host cells. Also disclosed is an infectious baculovirus comprising the artificial chromosome and a cell containing the chromosome. Also disclosed is an insect cell which expresses the LEF-8 gene in the absence of a baculovirus chromosome. Also disclosed is a method to express heterologous proteins in eukaryotic cells using the baculovirus artificial chromosome.

Baculovirus artificial chromosomes and methods of use

An expression cassette useful for the secretion of a heterologous protein from eukaryotic cells comprising a DNA sequence encoding a promoter functionally linked to a DNA sequence coding for the juvenile hormone esterase gene which is linked in frame to a heterologous gene is disclosed. Also disclosed is a method of secreting heterologous proteins in eukaryotic cells.

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Calgary, Canada

Patrick J Farrell