Polyplus-transfection Sa

Centre National De La Recherche Scientifique

Universitéde Strasbourg

Polyplus Transfection

Patrick Erbacher

Jean-Paul Behr

Anne-Laure Bolcato Bellemin

Nathalie Lenne

Patrick Neuberg

Valérie Toussaint Moreau

Fabrice Stock

Nadia Hafdi

Abdennaji Adib

Sébastien Franck

Elodie Wohlfrom

The invention relates to compositions comprising double-stranded oligonucleotides of identical or different sequences and/or length, said oligonucleotides having sequencesNN. . . NN. . . Nwherein&#x2014;N. . . Nis half of a double-stranded 19-28 mer oligonucleotide of sequence complementary to a target nucleic acid sequence present in a living cell, and&#x2014;N. . . Nis a 3-50 mer overhang of sequence allowing oligomerization of said double-stranded oligonucleotide. Compositions of transfection comprising said oligonucleotide compositions and there used for therapeutical application.

Oligonucleotides for RNA interference and biological applications thereof

The invention relates to compositions comprising double-stranded oligonucleotides of identical or different sequences and/or length, said oligonucleotides having sequencesNN. . . NN. . . Nwherein&#x2014;N. . . Nis half of a double-stranded 19-28mer oligonucleotide of sequence complementary to a target nucleic acid sequence present in a living cell, and&#x2014;N. . . Nis a 3-50mer overhang of sequence allowing oligomerization of said double-stranded oligonucleotide. Compositions of transfection comprising said oligonucleotide compositions and there used for therapeutical application.

The invention relates to a method for manipulating, isolating, detecting or amplifying a target nucleic acid in a sample by hybridization with an oligonucleotide-oligocation conjugate, comprising allowing said nucleic acid to react with an oligonucleotide-oligocation conjugate comprising at least A1 and Bj linked together directly or via a linker, wherein. A, is an i-mer oligonucleotides, with i=3 to 50, where Ai is an oligomer with naturally or non naturally occurring nucleobases and/or pentafuranosyl groups and/or native phosphodiester bonds, optionally comprising a marker group. Bj is a j-mer organic oligocation moiety, with j=1 to 50, where B is &#x2014;HPO&#x2014;R&#x2014;(NH&#x2014;R)&#x2014;NH&#x2014;R&#x2014;O&#x2014;, where R, Rand Rare lower alkylene, identical or different, NH&#x2014;R2 moieties being identical or different when n is &#x3e;1; HPO&#x2014;R&#x2014;CH(X)&#x2014;R&#x2014;O&#x2014;, where Ri and R3, identical or different, are lower alkylene and X is putrescine, spermidine or spermine residue.

Method for hybridizing nucleic acids

The invention relates to compositions of transfection comprising an oligonucleotide and an amphiphilic cationic molecule of formula (I) wherein, &#x2014;X is N&#x2014;R, S or O, Rbeing a C1-C4 alkyl radical or an hydroxylated C3-C6 alkyl radical, Rand R, identical or different, represent H or a C1-C4 alkyl radical, or Rand Rare linked together to form a saturated or unsaturated cycle or a heterocycle having 5 or 6 elements, E is a C1-C5 alkyl spacer, Rand R, identical or different, represent saturated or unsaturated, linear or branched, C10-C36 hydrocarbon or fluorocarbon chains, optionally comprising C3-C6 cycloalkyl, A&#x2212; is a biocompatible anion. The invention relates to compositions active for oligonucleotides delivery into eukaryotic cells in culture, ex vivo or in vivo. The invention relates to compositions of transfection comprising an oligonucleotide active for RNA interference. Such compositions can be used as tools for biological studies or as drugs for therapies.

Compositions for transfection of oligonucleotides active for gene silencing and their biological and therapeutical applications

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Benfeld, France

Patrick Erbacher