Yeda Research and Develpment & Co. Ltd

Patricia Orchansky

Menachem Rubinstein

Daniela Novick

Dina G Fischer

Analysis of [.sup.125 I]. interferon gamma cross-linked to its receptor on various human cells by SDS-PAGE revealed that there are at least three different types of human interferon gamma receptors. In WISH, HeLa, FS11 and the other tissue cells an Mr 90,000-105,000 receptor was found. In monocytes and in the myeloid cell line KG-1 an Mr 140,000 receptor was found while in Daudi lymphoblastoid cells an Mr 95,000-115,000 receptor was found. The various receptors were isolated from these cells by extraction followed by affinity chromotography on an immobilized interferon gamma column. The resulting purified preparations retained their original affinity for interferon gamma and were used for immunizing mice and subsequent development of highly specific antibodies.

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Rehovot, Israel

Patricia Orchansky