Institut National De La Sante Et De La Recherche Medicale

Institut Gustave Roussy

Université Paris-Saclay

Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement

Maat Pharma

Institut De La Recherche Agronomique

Patricia Lepage

Laurence Zitvogel

Ivo Gomperts Boneca

Sophie Viaud

Romain Daillere

Carole Schwintner

Hervé Affagard

Joël Dore

Catherine Juste

Fernanda Fonseca

Christel Maillet

Sylvie Rabot

Hervé Blottiere

The present invention provides methods for determining if a patient is likely to benefit from a cancer treatment, by determining if said patient has a gut dysbiosis with an over representation of certain bacterial species. The present invention also provides probiotic strains to improve the efficacy of a cancer treatment, especially chemotherapy, in patients in need thereof.

Microbiota composition, as a marker of responsiveness to chemotherapy, and use of microbial modulators (pre-, pro- or synbiotics) for improving the efficacy of a cancer treatment

The present invention relates to a method for preparing a fecal 5 microbiota sample of a donor subject. Said method includes the following steps: a) collecting at least one fecal microbiota sample from the donor subject; b) within a period of less than 5 minutes after collecting the sample, placing said sample obtained in step a) in an oxygen-tight collection device; c) mixing the sample obtained in step b) with at least one aqueous saline 10 solution containing at least one cryoprotectant and/or a filling agent; d) optionally, filtering the mixture obtained in step c), in particular by means of a filter comprising pores having a diameter of less than or equal to 0.7 mm, preferably less than or equal to 0.5 mm; and e) storing the mixture obtained in step c) or d) by freezing said mixture at a temperature 15 of between &#x2212;15&#xb0; C. and &#x2212;100&#xb0; C. Steps b) to e) are carried out in anaerobiosis.

Method of preparing a faecal microbiota sample

Growing community of inventors

Chatillon, France

Patricia Lepage