Eli Lilly and Company

Patricia J Solenberg

Richard H Baltz

Patti J Treadway

Jo Ann Hoskins

Nancy A Schaus

The invention provides isolated nucleic acid compounds encoding a glycosyltransferase enzyme ofAlso provided are vectors carrying genes that encode said enzyme, transformed heterologous host cells for expressing said enzyme, and methods for producing glycopeptide compounds using the cloned genes that encode said enzyme.

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The invention provides isolated nucleic acid compounds encoding a glycosyltransferase enzyme of . Also provided are vectors carrying genes that encode said enzyme, transformed heterologous host cells for expressing said enzyme, and methods for producing glycopeptide compounds using the cloned genes that encode said enzyme.

Glycosyltransferase gene gtfE from Amycolatopsis oreintalis

The invention provides isolated nucleic acid compounds encoding a glycosyltransferase enzyme of,Also provided are vectors carrying genes that encode said enzyme, transformed heterologous host cells for expressing said enzyme, and methods for producing glycopeptide compounds using the cloned genes that encode said enzyme.

Glucosyltransferase gene gtfe from Amycolatopsis orientalis

The invention provides isolated nucleic acid compounds encoding the glycosyltransferase protein GtfA of Amycolatopsis orientalis. Also provided are vectors carrying the gtfA gene, transformed heterologous host cells for expressing the GtfA protein, and methods for producing glycopeptide compounds using the cloned gtfA gene.

Glycosyltransferase gene gtfA from Amycolatopsis orientalis

The invention provides isolated nucleic acid compounds encoding the glycosyltransferase protein GtfC of Amycolatopsis orientalis. Also provided are vectors carrying the gtfC gene, transformed heterologous host cells for expressing the GtfC protein, and methods for producing glycopeptide compounds using the cloned gtfC gene.

Glycosyltransferase gene gtfC from Amycolatopsis orientalis

The invention provides isolated nucleic acid compounds encoding the glycosyltransferase protein GtfD of Amycolatopsis orientalis. Also provided are vectors carrying the gtfD gene, transformed heterologous host cells for expressing the GtfD protein, and methods for producing glycopeptide compounds using the cloned gtfD gene.

Glycosyltransferase gene GFTD from amycolatopsis orientalis

The invention provides isolated nucleic acid compounds encoding the glycosyltransferase protein GtfB of Amycolatopsis orientalis. Also provided are vectors carrying the gtfB gene, transformed heterologous host cells for expressing the GtfB protein, and methods for producing glycopeptide compounds using the cloned gtfB gene.

Glycosyltransferase gene gtfB from Amycolatopsis orientalis

Provided herein is a method to produce knockout mutations at targeted sites in the genome of Streptococcus pneumoniae.

Method for knockout mutagenesis in Streptococcus pneumoniae

The invention provides isolated nucleic acid compounds encoding a glycosyltransferase enzyme of Amycolatopsis orientalis. Also provided are vectors carrying genes that encode the enzyme, transformed heterologous host cells for expressing the enzyme, and methods for producing glycopeptide compounds using the cloned genes that encode the enzyme.

Glucosltransferase gene GTFE from amycolatopsis orientalis

The invention provides isolated nucleic acid compounds encoding a glycosyltransferase enzyme of Amycolatopsis orientalis. Also provided are vectors carrying genes that encode said enzyme, transformed heterologous host cells for expressing said enzyme, and methods for producing glycopeptide compounds using the cloned genes that encode said enzyme.

Glucosyltransferase gene GTFE from amycolatopsis orientalis

Glycosyltransferase gene GtfA from Amycolatopsis orientalis

Glycosyltransferase gene GtfC from Amycolatopsis orientalis

Glycosyltransferase gene gtfD from amycolatopsis orientalis

Glycosyltransferase gene gtfb from Amycolatopsis orientalis

A novel method for isolating transposable elements was used to isolate an approximately 1.6 kb insertion sequence from Streptomyces. The method entails transforming a cell with a plasmid containing a repressor gene, so that the introduction of a transposable element into the gene allows the expression of a selectable marker in a second host cell. The novel insertion sequence isolated from Streptomyces lividans CT2 has been designated IS493.

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Indianapolis, IN, United States of America

Patricia J Solenberg