Servicio Galego De Saude (sergas)

Fundación Profesor Novoa Santos

Servizo Galego De Saude

Patricia García Fernández

María Merino Carballeira

Alejandro Beceiro Casas

María Clara Póvoa Cabral

Germán Bou Arévalo

Astrid Pérez Gómez

The present invention refers to a method for the production of live attenuated bacterial strains, suitable as vaccine candidates, comprising the steps of:

Live attenuated vaccines

The present invention refers to a method for the production of live attenuated bacterial strains, suitable as vaccine candidates, comprising the steps of: A. providing a bacterial strain capable of expressing glutamate racemase and possibly D-amino acid transaminase and comprising a peptidoglycan cell wail, and B. inactivating the gene or genes encoding for the glutamate racemase enzyme and, if needed, the gene or genes encoding for the enzyme D-amino acid transaminase in such way that the bacterial strain is no longer capable of expressing a functional glutamate racemase and/or a functional D-amino acid transaminase; wherein the inactivation of said genes causes said bacterial strain to be auxotrophic for D-glutamate.

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A Coruña, Spain

Patricia García Fernández