Berkeley Lights, Inc.

Patricia A Dyck

J Tanner Nevill

Jason M McEwen

Volker L S Kurz

Gregory G Lavieu

Xiao Guan Radstrom

Annamaria Mocciaro

Magali Soumillon

Ravi K Ramenani

Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.

Methods, systems and devices for selection and generation of genome edited clones

Growing community of inventors

San Francisco, CA, United States of America

Patricia A Dyck