Centre National De La Recherche Scientifique

Université´de Rouen

Medicago Inc.

Institut National De La Recherche Agronomique (inra)

Institut Francais De Recherche Pour L'exploitation De La Mer (ifremer)

Algenics

L'institut Francais De Recherche Pour L'exploitation De La Mer (ifremer)

Université De Rouen Normandie

Institut Francais De Recherche Pour L'exploitation De La Mer

Christophe Rihouey

Marc-andre D'aoust

Louis-philippe Vezina

Veronique Gomord

Muriel Bardor

Stephanie Aquin

Patrice Lerouge

Marc-Andre D'Aoust

Louis-Philippe Vezina

Véronique Gomord

Marc-André D'Aoust

Jean-Paul Cadoret

Thomas Paccalet

Loïc Faye

Stéphanie Aquin

Carole Burel

Aude Carlier

Loic Faye

Philippe Roche

Jean Louis Denarie

Fabienne Chantal Maillet

Jean-Claude Adrien Prome

Georges Truchet

Catherine Faucher

Rémy Michel

Bérangère Baiet

Bruno Saint-Jean

Romain Louvet

Florian Maury

Marc-André D'AOUST

Estelle Marquet-Blouin

Loiec Faye

Marc-Andre D'aoust

Christophe Sourrouille

A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.

Synthesis of sialic acid in plants

Transformedincluding a nucleic acid sequence operatively linked to a promoter, wherein the nucleic acid sequence encodes an N-acetylglucosaminyltransferase I and/or an &#x3b1;-Mannosidase II and wherein at least one &#x3b2;-N-acetylglucosaminidase of the transformedhas been inactivated. A method for producing a glycosylated polypeptide includes the steps of (i) culturing a transformedas defined previously and (ii) purifying the polypeptide that is expressed and glycosylated in the transformed. The use of such a transformedfor producing a glycosylated polypeptide is also described.

[object Object]

A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5 Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.

Transformed microalgae capable of expressing glycosylated polypeptides and methods for producing said transformed microalgae and producing glycosylated polypeptides.

Production of glycosylated polypeptides in micro algae

An essentially pure substance has a structure of a Nod factor or one of its analogues. The Nod factor is characterized by the fact that its biosynthesis is controlled by at least one nodulation gene (nodA,B,C) common to the Rhizobiaceae, in particular to the genera Rhizobium, Bradyrhizobium, Sinorhizobium and Azorhizobium. This substance consists of a lipo-oligosaccharide which is not a derivative of the exopolysaccharides and which has the general formula (I). In formula (I), the Nod factor of which it has the structure is a plant-specific symbiotic signal and is capable of enhancing the capacity of the bacteria of infect the host plant with which it is associated and/or of accelerating the formation of nodules on the host plant with which it is associated and/or of inducing the transcription of symbiotic genes of the leguminoseae. Applications to the treatment of plants and as an active therapeutic agent in humans and animals. G stands for a hexosamine variously substituted, for example by an acetyl group on the nitrogen, a sulphate group, an acetyl group and/or an ether group on oxygen R.sub.1, R.sub.2, R.sub.3, R.sub.5, R.sub.6, R.sub.7, which may be identical or different, stand for H, CH.sub.3 CO--, C.sub.x H.sub.y CO-- where X is a whole number between 0 and 17 and Y is a whole number between 1 and 35, or any other acyl group, for example a carbamyl groups, R.sub.4 stands for a saturated or mono-, di or tri-unsaturated aliphatic chain containing at least 12 carbon atoms and n is a whole number between 1 and 4.

Substance with lipo-oligosaccharide structure capable of acting as

Growing community of inventors

Grand Couronne, France

Patrice Lerouge