Bio-rad Laboratories, Inc.

Harvard College

Centre National De La Recherche Scientifique

Gnubio, Inc.

Abate, Adam

Kiani, Sepehr

Esmail, Adnan, Moez

Mary, Pascaline

Hung, Tony

Pascaline Mary

Tony Hung

Adam R Abate

Sepehr Kiani

Adnan Moez Esmail

Tal Raz

David A Weitz

John Healy

Scott Powers

Jason Hung

John Lawrence Emhoff

Joshua Blouwolff

Patrick Tabeling

Fabrice Monti

Marie-Claude Potier

Luce Dauphinot

Nadege Bois

The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc. of a first fluid being urged into and/or out of a second fluid can be controlled by controlling various properties of the fluid and/or a fluidic droplet, for example curvature of the fluidic droplet, and/or controlling the applied electric field.

Fluid injection

The present invention relates to methods of target nucleic acid amplification and uses thereof in methods of sequencing, or other down-stream applications such a genotyping or cloning. More specifically, the methods relate to methods of target nucleic acid amplification through the use of a universal hairpin primer.

Universal hairpin primers

Methods and systems for manipulating drops in microfluidic channels are provided.

Microfluidic devices and methods of their use

The present invention generally pertains to a system for performing injection of multiple substantially controlled volumes into or out of a droplet, and methods and kits comprising the same. The system of the present invention comprises at least one microfluidic channel, one or more injection channels, an injection inlet associated with each of the one or more injection channels, and a mechanism for disrupting an interface between a droplet and a fluid and/or emulsion, wherein the at least one microfluidic channel comprises one or more droplets are flowing therein, and wherein each of the one or more injection channels comprises at least one fluid and/or emulsion therein.

Injection of multiple volumes into or out of droplets

A microfluidic device and method for producing and collecting single droplets of a first fluid, the device including a microfluidic platform having at least a droplet microchannel wherein is produced a flow of single droplets of the first fluid dispersed in a second fluid immiscible with the first fluid, the droplet microchannel having at least one inlet extremity and at least one outlet extremity for distributing the flow of droplets, the device further including:

Microfluidic device for production and collection of droplets of a fluid

The present invention generally pertains to methods for detecting the presence or absence of a particular nucleic acid sequence. The present invention generally relates to incorporating a detector into a target nucleic acid, adding an oligonucleotide probe, polymerase enzyme and an inhibitor to the reaction, and detecting interference of the oligonucleotide probe with the inhibitor as an indication of the presence of a particular target nucleic acid sequence as well as kits encompassing the same.

Nucleic acid target detection using a detector, a probe and an inhibitor

The present invention provides a microfluidic system, method and kit for performing assays. The system may comprise a microfluidic device and a detector, wherein the assay yields results that may be read by a detector and analyzed by the system. The assay may comprise one or more chemical or biological reaction against, or performed on, a sample or multiple samples. The sample(s) may become larger and/or smaller during the performance of the assay. The sample(s) may be present within a vehicle, or on a carrier within a vehicle, in the microfluidic device, and wherein the vehicle may become larger and/or smaller during the performance of the assay. The assay may be a cascading assay comprising a series of multiple assays, wherein each assay may be the same or different, and wherein each assay in the series of multiple assays may further comprise one or more process or step.

Integrated microfluidic system, method and kit for performing assays

The present invention generally pertains to methods and kits for managing the variation in spectroscopic intensity measurements through the use of a reference component. The reference component may comprise a reference spectroscopic substance and may be contained together with a sample of interest in a sample to be tested, wherein the sample of interest may comprise a sample spectroscopic substance. Each sample to be tested may be uniquely identified and, hence, 'barcoded' by combinations of different colors and concentrations of spectroscopic substances, contained therein.

Managing variation in spectroscopic intensity measurements through the use of a reference component

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Cambridge, MA, United States of America

Pascaline Mary