The Chemo-sero-therapeutic Research Institute

Glaxosmithkline Biologicals Sa

Km Biologics Co., Ltd.

Pascal Charles Louis Gerkens

Yoshinobu Miyatsu

Kenjiro Kawatsu

Tatsuya Kasugaya

Tetsuro Tanabe

Michele Therese Rita Lecocq

Jean-Francois Jose Alain Marie Ghislain Leruse

A method of producing a virus in cell culture comprising at least the steps of a) providing a population of cells cultured in a cell culture medium, b) infecting the population of cells by i. inoculating the population with the virus, and ii. incubating the inoculated population so as to allow the virus to replicate and propagate, c) collecting the produced virus, thereby providing a viral harvest, and d) purifying the virus, wherein a power density of at least 15 W/m, at least 30 W/m, at least 60 W/m, at least 100 W/m, at least 120 W/mis applied to the cell culture at least during step b).

Method of viral purification

Growing community of inventors

Rixensart, Belgium

Pascal Charles Louis Gerkens