The United States of America As Represented by the Administrator of the

Ecova, Inc.

Sbp Technologies, Inc.

Parmely H Pritchard

Michael J Nelson

Al Willis Bourquin

James G Mueller

Suzanne Lantz

A process for remediating contaminated soil or groundwater using a strain of Pseudomonas paucimobilis designated NRRL B-18512 is disclosed. Further, the process is a two stage sequential process wherein in the first stage bacteria which degrade low molecular weight polycyclic aromatic hydrocarbons, heterocyclic organic compounds and phenolics are used in a pretreatment step. However, the remaining high molecular weight compounds are treated in a second step of the process with the Pseudomonas paucimobilis strain since the strain is not inhibited by low molecular weight compounds because these are removed in the first pretreatment step. Therefore, repeated inoculation of the strain is not necessary and the overall process disclosed eliminates the inhibitory effects of the low molecular weight compounds. A Microorganism designated CRE1-13 is a useful strain for the pretreatment step in order to enhance activity and viability of the strain, Pseudomonas paucimobilis designated NRRL B-18512, useful for removing the remaining high molecular compounds in creosote from soil or groundwater. In addition Pseudomonas sp. strain SR-3 is also useful for removing high molecular weight compounds in creosote.

Bioremediation of soil or groundwater contaminated with compounds in

A method of degrading halogenated aliphatic hydrocarbons is disclosed comprising incubating microorganisms capable of degrading halogenated aliphatic hydrocarbons by an aromatic degradative pathway together with the halogenated aliphatic hydrocarbons under conditions such that said aromatic degradative pathway is active.

Biodegradation of aliphatic chloroethylene compounds

A method of degrading chloroethylene compounds is disclosed comprising incubating microorganisms capable of degrading chloroethylene compounds by an aromatic degradative pathway together with the chloroethylene compounds under conditions such that the aromatic degradative pathway is active.

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Gulf Breeze, FL, United States of America

Parmely H Pritchard