Biomerieux, Inc.

Parampal Deol

Douglas Lovern

Yoany Portilla

Leticia Barton

John D Walsh

Jones M Hyman

Maud Arsac

Victoria Girard

Erik Moreno

Elizabeth Miller

Heather Totty

Amber Gates

Sandrine Mailler

Eric Miller

John Walsh

The present invention is directed to a method and kit for inactivation of acid-fast bacteria. In some embodiments, the method may include: transferring a sample from a liquid culture containing acid-fast bacteria to a first tube, wherein the first tube comprises a body, a first end to the body having an opening, and a second end to the body having a frustoconical portion ending in a concave tip; centrifuging the tube to pellet the acid-fast bacteria in the concave tip and subsequently decanting a supernatant; resuspending the acid-fast bacteria pellet in alcohol; transferring the suspension to a second tube containing beads; agitating the second tube to disrupt acid-fast bacteria cells; and incubating the suspension to inactivate the acid-fast bacteria in the test sample. The method may also include identifying the acid-fast bacteria with mass spectrometry.

Methods for inactivation and extraction of acid-fast bacteria from liquid media for characterization and/or identification using mass spectrometry

The present invention relates to an improved culture medium and method for the enhanced growth and detection ofgrowth. The invention further relates to an improved mycobacterial reagent system or kit that can be used for the enhanced growth and detection of

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The present invention is directed to a method for inactivation and/or extraction of acid-fast bacteria (e.g.,or), the method comprising the following sequential steps: (a) acquiring a test sample known to contain or that may contain acid-fast bacteria and suspending the test sample in a container containing ethanol and beads; (b) bead beating and/or vortexing the container to break up clumps and/or disrupt acid-fast bacteria cells in the container; and (c) subsequently incubating the suspension for at least about 3 minutes at room temperature to inactivate any acid-fast bacteria contained in the test sample. In accordance with the present invention, the test sample can subsequently be pelleted by centrifugation, resuspended with formic acid, acetonitrile added, and subjected to mass spectrometry for characterization and/or identification of the acid-fast bacteria.

Methods for inactivation and extraction of acid-fast bacteria for characterization and/or identification using mass spectrometry

Method and culture medium for enhanced detection of mycobacterium

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Raleigh, NC, United States of America

Parampal Deol