Fondazione Istituto Italiano Di Tecnologia

The General Hospital Corporation

Consiglio Nazionale Delle Ricerche

Paolo Netti

Rakesh K Jain

Roberto Cingolani

Pier Paolo Pompa

Raffaele Vecchione

Pietro Ferraro

Robert J Melder

Andrea Finizio

Simonetta Grilli

Veronica Vespini

Stefania Sabella

Anna Aliberti

Sara Coppola

Eliana Esposito

Filippo Causa

Gabriel Helmlinger

Hera Lichtenbeld-Dubois

Edmondo Battista

Enza Torino

Angela Maria Cusano

Renato De Alteriis

Maria Ada Malvindi

Costantino Casale

Giorgia Imparato

Francesco Urciuolo

Sara Scamardella

The present disclosure relates to a process for the preparation of core-shell particles by the coacervation method encapsulating contrast agents for multimodal imaging. The process consists in: a. Providing a water in oil emulsion of a biocompatible polyelectrolyte polymer. b. Providing an aqueous solution of a biocompatible polyelectrolyte polymer having opposite charges of the polyelectrolyte of step a). c. Adding a crosslinking agent to the primary emulsion and the secondary solution. d. Adding at least a tracer independently to the primary emulsion or the secondary solution or emulsion. e. Adding the secondary aqueous solution to the primary emulsions and occurring of the complex coacervation leading to the separation of the coacervate particles. f. Optionally absorb a further tracer into the nanoparticles The disclosure also relates to the coacervates obtained by the above described process and their use as probe for multimodal imaging in the diagnostic field.

Process for the preparation of double crosslinked core-shell polymeric nanoparticles for multimodal imaging and theranostic applications

Method for producing shaped polymeric microparticles of non-spherical shape, comprising the steps of: placing one or more microparticles of substantially spherical shape in a respective micro-cavity of a mold having the desired non-spherical shape; subjecting said microparticles to softening by exposure to a solvent or mixture of solvent/non-solvent, in the liquid or vapor state, adapted to plasticize the polymeric material constituting said microparticles, and possibly assisting the solvent plasticization process by heat treatment, not excluding the possibility, in less critical cases in terms of conservation of the microstructure, of carrying out heat treatment exclusively, at a temperature not exceeding 40% of the glass transition temperature of the polymer material; and removing said microparticles from the mold cavities.

Method for producing shaped polymeric microparticles

There is disclosed a kit for detecting a single strand target nucleotide sequence comprising:

Probe kit for detecting a single strand target nucleotide sequence

There is described a method for making an array of micro-needles, comprising the steps of:

Method for making an array of micro-needles

Cells in a matrix or in the matrix in a vessel are grown to form a multicellular aggregate. Pressure is exerted on the growing cells by the matrix or the matrix together with the vessel due to growing cellular mass displacing the matrix. A value representing pressure exerted on the cells is calculated and the pressure is modulated to control growth of the multicellular aggregate, or to produce a multicellular aggregate of a pre-selected size or a pre-selected size and shape. Matrices include agarose, alginate and collagen gels, and the pressure exerted on the cells can be non-isotropic. The cells may be tumor cells, or liver, pancreatic, brain, skin, bone or muscle cells, and cell growth can be in vitro or in vivo. When collagen forms the matrix, the matrix may contain glycosaminoglycan.

Modulation of multicellular aggregates by pressure from growth in a matrix

The invention provides a method of enhancing the amount of a pharmaceutical composition delivered to a target tissue site in a mammal, by creating a transient differential between tile hydrostatic pressure in the target site and a region near the target tissue site whereby the composition is transported toward the site. An apparatus for performing the method is provided. In one form that apparatus includes a pharmaceutical reservoir, pump, and an agent reservoir and pump.

Method of enhancing delivery of a pharmaceutical formulation

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Naples, Italy

Paolo Netti