Tate & Lyle Ingredients Americas LLC

Universitá Degli Studi Di Milano-Bicocca

Galatea Biotech S.r.l.

Paola Branduardi

Danilo Porro

Diethard Mattanovich

Michael Sauer

Nadia Maria Berterame

Minoska Valli

Stefano Bertacchi

Disclosed is the production by fermentation of poly D-lactic acid (PDLA) and poly L-lactic acid (PLLA). In particular, there is provided engineered (prokaryotic or eukaryotic) cells for the direct synthesis of PLLA polymers and engineered eukaryotic cells for the direct synthesis of PDLA polymers starting from a carbon source, including residual biomasses of the different production chains.

Cell able to produce poly l-lactic acid

A method of producing an organic acid by staining a yeast population with a stain capable of internal pH (pH)-dependent fluorescence, to yield a stained yeast population; determining a gate pH and a corresponding fluorescence parameter of the stained yeast population; and sorting the cells of the stained yeast population such that the cells having a pHabove the gate pH are retained and the cells having a pHbelow the gate pH are discarded, to yield a yeast population for the production of the organic acid. Also, a method of producing an organic acid by performing the above steps, followed by isolating individual cells of the yeast population, to yield individual yeast cells for the production of an organic acid; culturing an individual yeast cell, to yield a cloned yeast population for the production of an organic acid; and incubating the cloned yeast population for the production of an organic acid in a medium containing an organic acid precursor, to produce the organic acid.

Methods for selecting a yeast population for the production of an organic acid and producing an organic acid

Growing community of inventors

Milan, Italy

Paola Branduardi