Pacific Biosciences of California, Inc.

Li-cor, Inc.

Pamela Sheaff

John G K Williams

Jiyan Chen

Gregory R Bashford

Dan Draney

Nara Narayanan

Bambi L Reynolds

John GK Williams

The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.

Flowcell systems for single molecule detection

Charge switch nucleotides

The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charged-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.

Charge-switch nucleotides

Growing community of inventors

Omaha, NE, United States of America

Pamela Sheaff