Georgia Tech Research Corporation

University of California

Pamela Peralta-Yahya

Stephen Sarria

Jay D Keasling

Souryadeep Bhattacharyya

Amy M Ehrenworth

Kuntal Mukherjee

Michael D Burkart

Taek Soon Lee

Adam Verga

Provided herein are biocatalysts and systems thereof for pterin-dependent enzymes and pathways and methods of making and using the same.

Pterin-dependent biocatalysts and uses thereof

The disclosure is directed to a method of engineering a heterologous thioesterase (TE) enzyme to interact with endogenous acyl-ACP proteins in order to produce molecules of interest in a bacterial host. The method can identify amino acids at the binding interface between the heterologous TE and the endogenous acyl-ACP that can be substituted so that the heterologous TE can better interact with the endogenous acyl-ACP. Mutant heterologous TE enzymes with improved interactions with the endogenous acyl-ACP are also provided.

Engineered thioesterase mutants and methods of using same to produce fatty acids

Provided herein are biocatalysts and systems thereof for pterin-dependent enzymes and pathways and methods of making and using the same. Provided herein in some embodiments are biocatalysts having a pterin source and a pterin-dependent enzymatic pathway biologically coupled to the pterin source. Tetrahydrobiopterin (referred to herein as BH4 or BH 4) can be the pterin source. The BH4 can be synthesized by a tetrahydrobiopterin synthesis pathway. The tetrahydrobiopterin synthesis pathway can include a GTP cyclohydrase; a pyruvoyl tetrahydropterin synthase; a sepiapterin reductase, and/or any combination thereof. The biocatalyst can further contain a pterin-dependent enzymatic pathway. The pterin-dependent enzymatic pathway can be amino acid mono-oxygenase, phenylalanine hydroxylase, tryptophan hydroxylase, tyrosine hydroxylase, nitric oxide synthase, alkylglycerol monooxygenase, and/or any combination thereof.

Provided herein are GPCR-based chemical biosensors that can have a sensing unit, a processing unit, and a response unit that can be used to detect a chemical of interest. Also provided herein are methods of making and using the GPCR-based chemical biosensors.

G-protein coupled receptor (GPCR)-based biosensors and uses thereof

The present invention provides for a modified host cell comprising a heterologous pinene synthase (PS), or enzymatically active fragment or variant thereof, and optionally a geranyl pyrophosphate synthase (GPPS), or enzymatically active fragment or variant thereof, or a fusion protein comprising: (a) a PS and (b) a GPPS linked by a linker.

Fusion proteins useful for producing pinene

Fuel compositions are provided comprising a hydrogenation product of a monocyclic sesquiterpene (e.g., hydrogenated bisabolene) and a fuel additive. Methods of making and using the fuel compositions are also disclosed. 

Growing community of inventors

Atlanta, GA, United States of America

Pamela Peralta-Yahya