Oy Alko Ab

Alko-Yhtiot Oy

Alko-Yhtiot Oy (Alko Group Ltd.)

Paivi Lehtovaara-Helenius

Merja Elisa Penttila

Jonathan Knowles

Tuula Teeri

Helena Nevalainen

Marja T Paloheimo

Sirkka Keranen

Sirpa Aho

Irma Salovuori

Sunee Nitisinprasert

Helena M Nevalainen

Functional derivatives of endoglucanase I (EGI), and the cloning and recombinant expression thereof, are described.

Yeast strains producing cellulolytic enzymes and methods and means for

Described are methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes. This is achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze 3-1,4-glucan substrates such as cellulose.

Method of making endoglucanase I

Methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes, achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze 3-1,4-glucan substrates such as cellulose.

DNA, vectors and transformed hosts encoding Trichoderma reesei

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Helsinki, Finland

Paivi Lehtovaara-Helenius