Centre National De La Recherche Scientifique

Université´ D'aix-marseille

Université De La Méditerranée

Pablo Gluschankof

Didier Raoult

Gilles Audoly

Najoua Ben M'Barek

Pierre Nicolas

Christine M Clamagirand

Hamadi Boussetta

Christelle Perrin-East

Sophie Gomez

Paul Cohen

Alain Morel

A method of determining sensitivity or resistance of isolates of HIV retroviruses to a molecule includes a) amplifying sequences coding for a protease of a retrovirus to be studied, with or without the or some of amino acid sequences situated upstream and downstream of a cleavage site of a precursor in which the amino acid sequences are situated, b) recombining fragments of DNA, a final product of the amplification, and an expression vector allowing expression of sequence coding for the protease of the retrovirus to be studied under control of a known inducible promoter through co-transformation of the vector and the DNA fragments with at least one yeast cell, c) culturing co-transformed yeast cell or cells to obtain a sufficient number of transformants to perform a sensitivity or resistance test, and recovering transformants issuing from the co-transformed cell, on any suitable medium, d) incubating the transformants in the presence of a molecule to be tested, e) qualitatively or quantitatively analyzing the living cells, and f) deducing the sensitivity or resistance phenotype.

Yeast-based assay for measuring the functional activity of an HIV-1 protease in response to an antiviral agent

A method for determining sensitivity or resistance of isolates of HIV (human immunodeficiency virus) retroviruses to chemical molecules having an inhibiting activity on a viral protease or to therapeutic treatments based on inhibitors of the viral protease, including causing cell lysis of at least one yeast by expression of the retrovirus protease.

Methods for determining human immunodeficiency virus type 2 (HIV-2) protease sensitivity or resistance to antivirals utilizing an inducible yeast expression system

The invention relates to a method for measuring the enzymatic activity of a convertase, wherein:

Method for the determination of the enzymatic activity of convertase

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Marseilles, France

Pablo Gluschankof