Institut National De La Sante Et De La Recherche Medicale

Assistance Publique-Hopitaux De Paris

Université Paris Est Creteil Val De Marne

Etablissement Francais Du Sang

Institut National De La Sante Et De La Recherche Medicale (Inserm)

Institut National Transfusion Sanguine

Pablo Bartolucci

Michael Marden

Frédéric Galacteros

Gaetana Vandemeulebrouck

Nicolas Hebert

France Noizat Pirenne

Marie Georgine Rakotoson

Laurent Kiger

Marie Cambot

France Pirenne

The aim of the present invention is to propose a novel method for spectral study of a biological fluid from an absorption spectrum of a sample of the biological fluid. The inventors have made an original use of spectroscopy in order to accurately determine the protein content of a biological sample, in particular for oxyhaemoglobin, methaemoglobin, bilirubin and the haem bound to serum albumin. Such a method can be used in a variety of applications concerning haem- or haemoprotein- related diseases, in particular diagnostic methods, treatment follow-up methods, methods for determining biomarkers or screening methods. Such a method is also advantageous for qualifying blood bags.

Method for spectral study of a biological fluid

The invention concerns a method for determining, by flow cytometry, the hemoglobin F (HbF) content of each erythroid cell of a set of erythroid cells. This method applies in particular to determining the HbF content of each red blood cell of a set of red blood cells. The invention also concerns a method for determining the amount of red blood cells transfused into a patient and for monitoring the therapeutic efficacy of a treatment for sickle cell disease or &#x3b2;-thalassemia.

Method for determining the haemoglobin F content of an erythroid cell

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L'Hay les Roses, France

Pablo Bartolucci