Ventrex Laboratories, Inc.

Vxr, Inc.

P Narayan Nayak

An apparatus and method for performing enzyme-linked immunological sandwich assays (ELISA) by affinity chromatography. Formation of the antibody-antigen-conjugate complex, and the development of a chromatophore indicator take place in a semi-automated, walk-away, fashion. The immunoassay apparatus comprises a chromatography tank, which further comprises a reaction compartment and a plurality of liquid solvent reservoirs, with a first-liquid reservoir being configured higher than a second-liquid reservoir. The apparatus allows automatic sequential and discrete movement of wash solution, followed by a color development solution, with unbound conjugate being substantially washed away from the immunological sandwich complex before the color development solution automatically comes into contact with the immunological complex.

Apparatus and method for performing immunoassays

This invention relates to devices and to methods, diagnostic reagents and kits embodying such devices for use in ligand/anti-ligand assays and the qualitative or semi-quantitative detection of a substance of interest thereby through use of light or photon detecting systems such as reflectometers, colorimeters, fluorimeters and the human eye. Such devices, methods and kits have applications in the biologic research and medical fileds for assaying drugs, hormones, peptides, proteins, enzymes, nucleic acids, antibodies, haptens, antibiotics, viri, infectious agents, tumor markers and the like, in the laboratory, the physicians' office, the veterinarian's office and the home.

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Yarmouth, ME, United States of America

P Narayan Nayak